Protein: P61088

UniprotKB AC UniprotKB ID Gene name Full name Species Curated set
P61088 (Uniprot) UBE2N_HUMAN UBE2N Ubiquitin-conjugating enzyme E2 N human No
Uniprot: The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. more..
MoonDB Dissimilar Functions
GO ID 1 Function 1 Module ID 1 GO ID 2 Function 2 Module ID 2 Association Probability (PrOnto) Interaction Probability (PrOnto)
GO:0007165 signal transduction 4 GO:0006725 cellular aromatic compound metabolic process 53 5.44e-126 5.27e-27
GO:0009056 catabolic process 125 GO:0009059 macromolecule biosynthetic process 192, 270 6.37e-04 1.88e-40
Biological Processes
Module ID (MoonGO) GO ID (BP) GO Name
4 GO:0035556 intracellular signal transduction
4 GO:0031399 regulation of protein modification process
4 GO:0009966 regulation of signal transduction
53 GO:0006139 nucleobase-containing compound metabolic process
53 GO:0016567 protein ubiquitination
53 GO:0060255 regulation of macromolecule metabolic process
79 GO:0007165 signal transduction
125 GO:0044248 cellular catabolic process
125 GO:0016567 protein ubiquitination
125 GO:0060255 regulation of macromolecule metabolic process
134 GO:0044267 cellular protein metabolic process
134 GO:0060255 regulation of macromolecule metabolic process
134 GO:0007165 signal transduction
172 GO:0060255 regulation of macromolecule metabolic process
192 GO:0034645 cellular macromolecule biosynthetic process
192 GO:0006464 cellular protein modification process
192 GO:0010467 gene expression
192 GO:0090304 nucleic acid metabolic process
192 GO:0009893 positive regulation of metabolic process
192 GO:0060255 regulation of macromolecule metabolic process
192 GO:0007165 signal transduction
204 GO:0044267 cellular protein metabolic process
207 GO:0007165 signal transduction
217 GO:0060255 regulation of macromolecule metabolic process
217 GO:0007165 signal transduction
270 GO:0034645 cellular macromolecule biosynthetic process
270 GO:0010467 gene expression
270 GO:0090304 nucleic acid metabolic process
270 GO:0032446 protein modification by small protein conjugation
270 GO:0060255 regulation of macromolecule metabolic process
270 GO:0007165 signal transduction
297 GO:0007165 signal transduction
331 GO:0016070 RNA metabolic process
331 GO:0006464 cellular protein modification process
331 GO:0010467 gene expression
331 GO:0060255 regulation of macromolecule metabolic process
331 GO:0019219 regulation of nucleobase-containing compound metabolic process
358 GO:0006464 cellular protein modification process
358 GO:0060255 regulation of macromolecule metabolic process
358 GO:0007165 signal transduction
359 GO:0044248 cellular catabolic process
359 GO:0000209 protein polyubiquitination
359 GO:0007165 signal transduction
375 GO:0070647 protein modification by small protein conjugation or removal
375 GO:0060255 regulation of macromolecule metabolic process
381 GO:0006464 cellular protein modification process
381 GO:0010467 gene expression
381 GO:0016310 phosphorylation
381 GO:0010604 positive regulation of macromolecule metabolic process
381 GO:0050790 regulation of catalytic activity
381 GO:0032268 regulation of cellular protein metabolic process
381 GO:0007165 signal transduction
425 GO:0006464 cellular protein modification process
474 GO:0044267 cellular protein metabolic process
474 GO:0090304 nucleic acid metabolic process
474 GO:0060255 regulation of macromolecule metabolic process
531 GO:0019538 protein metabolic process
575 GO:0016567 protein ubiquitination
575 GO:0060255 regulation of macromolecule metabolic process
596 GO:0006464 cellular protein modification process
692 GO:0016070 RNA metabolic process
692 GO:0044271 cellular nitrogen compound biosynthetic process
692 GO:0010467 gene expression
692 GO:0019538 protein metabolic process
692 GO:2000112 regulation of cellular macromolecule biosynthetic process
763 GO:0060255 regulation of macromolecule metabolic process
774 GO:0006464 cellular protein modification process
774 GO:0060255 regulation of macromolecule metabolic process
775 GO:0044267 cellular protein metabolic process
775 GO:0010467 gene expression
775 GO:0006139 nucleobase-containing compound metabolic process
775 GO:0060255 regulation of macromolecule metabolic process
803 GO:0007166 cell surface receptor signaling pathway
803 GO:0044267 cellular protein metabolic process
803 GO:0071310 cellular response to organic substance
803 GO:0035556 intracellular signal transduction
803 GO:0009966 regulation of signal transduction
807 GO:0070647 protein modification by small protein conjugation or removal
824 GO:0044267 cellular protein metabolic process
824 GO:0010467 gene expression
824 GO:0007165 signal transduction
Cellular Components
Module ID (MoonGO) GO ID (CC) GO Name
4 GO:0005829 cytosol
4 GO:0005634 nucleus
53 GO:0005829 cytosol
53 GO:0005654 nucleoplasm
79 GO:0005634 nucleus
125 GO:0005829 cytosol
125 GO:0005634 nucleus
134 GO:0005829 cytosol
134 GO:0005634 nucleus
192 GO:0005829 cytosol
192 GO:0005654 nucleoplasm
204 GO:0005634 nucleus
207 GO:0005829 cytosol
207 GO:0005654 nucleoplasm
217 GO:0005829 cytosol
217 GO:0043232 intracellular non-membrane-bounded organelle
227 GO:0005634 nucleus
270 GO:0005829 cytosol
270 GO:0070013 intracellular organelle lumen
270 GO:0005634 nucleus
297 GO:0005634 nucleus
331 GO:0005654 nucleoplasm
358 GO:0012505 endomembrane system
358 GO:0005615 extracellular space
358 GO:0005886 plasma membrane
358 GO:0031982 vesicle
359 GO:0005634 nucleus
368 GO:0005829 cytosol
368 GO:0005634 nucleus
368 GO:0031982 vesicle
375 GO:0005829 cytosol
375 GO:0005634 nucleus
381 GO:0005829 cytosol
381 GO:0005634 nucleus
425 GO:0005634 nucleus
575 GO:0005654 nucleoplasm
596 GO:0005634 nucleus
692 GO:0031981 nuclear lumen
721 GO:0043232 intracellular non-membrane-bounded organelle
721 GO:0005634 nucleus
763 GO:0005634 nucleus
774 GO:0005634 nucleus
775 GO:0005829 cytosol
775 GO:0005634 nucleus
803 GO:0016021 integral component of membrane
803 GO:0005886 plasma membrane
GO ID (BP) GO Name Evidence Code (GO EC)
GO:0000187 activation of MAPK activity TAS
GO:0000724 double-strand break repair via homologous recombination IMP
GO:0000729 DNA double-strand break processing IMP
GO:0002223 stimulatory C-type lectin receptor signaling pathway TAS
GO:0006282 regulation of DNA repair TAS
GO:0006301 postreplication repair IMP
GO:0006303 double-strand break repair via nonhomologous end joining TAS
GO:0006464 cellular protein modification process TAS
GO:0006508 proteolysis TAS
GO:0006511 ubiquitin-dependent protein catabolic process IEA
GO:0007254 JNK cascade TAS
GO:0016567 protein ubiquitination TAS
GO:0016574 histone ubiquitination IMP
GO:0031058 positive regulation of histone modification IMP
GO:0033182 regulation of histone ubiquitination IMP
GO:0038095 Fc-epsilon receptor signaling pathway TAS
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB signaling TAS
GO:0045739 positive regulation of DNA repair IMP
GO:0050852 T cell receptor signaling pathway TAS
GO:0051092 positive regulation of NF-kappaB transcription factor activity TAS
GO:0051443 positive regulation of ubiquitin-protein transferase activity IMP
GO:0070423 nucleotide-binding oligomerization domain containing signaling pathway TAS
GO:0070534 protein K63-linked ubiquitination IMP
GO:0070911 global genome nucleotide-excision repair TAS
GO ID (CC) GO Name Evidence Code (GO EC)
GO:0000151 ubiquitin ligase complex IDA
GO:0001650 fibrillar center IDA
GO:0005634 nucleus IDA
GO:0005654 nucleoplasm TAS
GO:0005737 cytoplasm IDA
GO:0005829 cytosol TAS
GO:0031372 UBC13-MMS2 complex IDA
GO:0035370 UBC13-UEV1A complex IDA
GO:0043234 protein complex IDA
GO:0070062 extracellular exosome IDA
GO ID 1 Component 1 GO ID 2 Component 2 Association Probability (PrOnto) Interaction Probability (PrOnto)
GO:0005634 nucleus GO:0070062 extracellular exosome 6.30e-13 4.54e-26
GO:0005654 nucleoplasm GO:0070062 extracellular exosome 3.37e-20 1.23e-35
Interactor Also a MoonDB EMF protein
TRI27_HUMAN Yes: P14373 (MoonDB)
XIAP_HUMAN Yes: P98170 (MoonDB)
BIRC7_HUMAN No
CHIP_HUMAN Yes: Q9UNE7 (MoonDB)
CALM1_HUMAN Yes: P0DP23 (MoonDB)
HG2A_HUMAN No
HGS_HUMAN No
RNF8_HUMAN No
TRIM1_HUMAN Yes: Q9UJV3 (MoonDB)
SIAH1_HUMAN Yes: Q8IUQ4 (MoonDB)
ARI2_HUMAN No
SH3R2_HUMAN No
TM189_HUMAN No
TRI50_HUMAN No
CCD69_HUMAN No
ACOX1_HUMAN No
AMFR_HUMAN No
AN13A_HUMAN No
AN13D_HUMAN No
APEX2_HUMAN No
ASCC2_HUMAN No
ATX3_HUMAN No
BAP1_HUMAN No
BFAR_HUMAN No
BIRC3_HUMAN No
BIRC8_HUMAN No
BRCA1_HUMAN No
CAPS2_HUMAN No
CAR14_HUMAN No
CBLB_HUMAN No
CEP85_HUMAN No
CF106_HUMAN No
CHFR_HUMAN No
CNOT4_HUMAN No
CREL1_HUMAN No
CUED1_HUMAN No
DBF4B_HUMAN No
DCNL1_HUMAN No
DCNL2_HUMAN No
DEN6B_HUMAN No
DNJB2_HUMAN No
DNJB6_HUMAN No
DP13A_HUMAN Yes: Q9UKG1 (MoonDB)
DTX1_HUMAN No
DZIP3_HUMAN No
EPHA1_HUMAN No
EPN1_HUMAN Yes: Q9Y6I3 (MoonDB)
F184A_HUMAN No
FAF2_HUMAN No
FKBP6_HUMAN No
G3V219_HUMAN No
GGA2_HUMAN No
GOLI_HUMAN No
GRAP1_HUMAN No
HBS1L_HUMAN No
HIP1R_HUMAN No
HIP1_HUMAN Yes: O00291 (MoonDB)
HOIL1_HUMAN No
IMPCT_HUMAN No
INT3_HUMAN No
KRT36_HUMAN No
LRSM1_HUMAN Yes: Q6UWE0 (MoonDB)
MARH5_HUMAN No
MARH7_HUMAN No
MFSD6_HUMAN No
MIB1_HUMAN No
MKRN3_HUMAN Yes: Q13064 (MoonDB)
NEPR1_HUMAN No
NEUL1_HUMAN No
NFX1_HUMAN No
NUDT6_HUMAN No
PELI1_HUMAN No
PELI2_HUMAN No
PJA2_HUMAN No
POLI_HUMAN No
Q6GX22_HUMAN No
Q8NEX1_HUMAN No
RC3H2_HUMAN No
RET2_HUMAN No
RFWD3_HUMAN No
RHBL4_HUMAN No
RIOK3_HUMAN No
RN103_HUMAN No
RN111_HUMAN Yes: Q6ZNA4 (MoonDB)
RN122_HUMAN No
RN125_HUMAN No
RN135_HUMAN No
RN152_HUMAN Yes: Q8N8N0 (MoonDB)
RN165_HUMAN No
RN167_HUMAN Yes: Q9H6Y7 (MoonDB)
RN182_HUMAN No
RNF11_HUMAN No
RNF13_HUMAN No
RNF34_HUMAN No
RNF43_HUMAN No
RNF4_HUMAN No
RO52_HUMAN No
SL9B2_HUMAN No
SMUF1_HUMAN No
THA_HUMAN No
TOPRS_HUMAN No
TRAF6_HUMAN Yes: Q9Y4K3 (MoonDB)
TRI11_HUMAN No
TRI18_HUMAN No
TRI23_HUMAN No
TRI25_HUMAN No
TRI32_HUMAN No
TRI52_HUMAN No
TRIM5_HUMAN Yes: Q9C035 (MoonDB)
TSN17_HUMAN No
UB2D3_HUMAN Yes: P61077 (MoonDB)
UB2E1_HUMAN Yes: P51965 (MoonDB)
UB2E2_HUMAN Yes: Q96LR5 (MoonDB)
UB2V1_HUMAN Yes: Q13404 (MoonDB)
UB2V2_HUMAN No
UBC_HUMAN Yes: P0CG48 (MoonDB)
ZNRF1_HUMAN No
ZNRF2_HUMAN No
ZNRF3_HUMAN No
ZNRF4_HUMAN No
PMID Article Title
8902611 Cloning and expression of cDNA encoding a human ubiquitin-conjugating enzyme similar to the Drosophila bendless gene product.
10089880 Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in assembly of novel polyubiquitin chains for DNA repair.
11473255 Crystal structure of the human ubiquitin conjugating enzyme complex, hMms2-hUbc13.
14562038 The Chfr mitotic checkpoint protein functions with Ubc13-Mms2 to form Lys63-linked polyubiquitin chains.
15489334 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
16112642 ISG15 modification of Ubc13 suppresses its ubiquitin-conjugating activity.
16122702 ISG15 modification of ubiquitin E2 Ubc13 disrupts its ability to form thioester bond with ubiquitin.
16215985 The RING finger protein RNF8 recruits UBC13 for lysine 63-based self polyubiquitylation.
16307917 Chaperoned ubiquitylation -- crystal structures of the CHIP U box E3 ubiquitin ligase and a CHIP-Ubc13-Uev1a complex.
17108083 Human SHPRH is a ubiquitin ligase for Mms2-Ubc13-dependent polyubiquitylation of proliferating cell nuclear antigen.
17130289 Human SHPRH suppresses genomic instability through proliferating cell nuclear antigen polyubiquitination.
17135271 Site-specific Lys-63-linked tumor necrosis factor receptor-associated factor 6 auto-ubiquitination is a critical determinant of I kappa B kinase activation.
18316726 Human HLTF functions as a ubiquitin ligase for proliferating cell nuclear antigen polyubiquitination.
18615712 Analysis of electrostatic contributions to the selectivity of interactions between RING-finger domains and ubiquitin-conjugating enzymes.
18719106 Polyubiquitination of proliferating cell nuclear antigen by HLTF and SHPRH prevents genomic instability from stalled replication forks.
19203578 The RIDDLE syndrome protein mediates a ubiquitin-dependent signaling cascade at sites of DNA damage.
19269966 Regulation of endoplasmic reticulum-associated degradation by RNF5-dependent ubiquitination of JNK-associated membrane protein (JAMP).
19340006 The ubiquitin ligase Triad1 inhibits myelopoiesis through UbcH7 and Ubc13 interacting domains.
19608861 Lysine acetylation targets protein complexes and co-regulates major cellular functions.
20061386 The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines.
20725033 Non-canonical inhibition of DNA damage-dependent ubiquitination by OTUB1.
21269460 Initial characterization of the human central proteome.
21512573 TRIM5 is an innate immune sensor for the retrovirus capsid lattice.
21659603 A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling.
22325355 OTUB1 co-opts Lys48-linked ubiquitin recognition to suppress E2 enzyme function.
22367539 The mechanism of OTUB1-mediated inhibition of ubiquitination.
22589545 Molecular insights into the function of RING Finger (RNF)-containing proteins hRNF8 and hRNF168 in Ubc13/Mms2-dependent ubiquitylation.
22693631 Covalent protein modification with ISG15 via a conserved cysteine in the hinge region.
25944712 N-terminome analysis of the human mitochondrial proteome.
No results found.
Domain Name Domain ID Source
UBQ-conjugat_E2 IPR000608 InterPro
UBQ-conjugating_enzyme/RWD IPR016135 InterPro
UBQ-conjugating_AS IPR023313 InterPro
JC4894 JC4894 PIR
UQ_con PF00179 Pfam
UBIQUITIN_CONJUGAT_1 PS00183 PROSITE
UBIQUITIN_CONJUGAT_2 PS50127 PROSITE
SSF54495 SSF54495 SUPFAM
UBCc cd00195 CDD