Protein: P14618

UniprotKB AC UniprotKB ID Gene name Full name Species Curated set
P14618 (Uniprot) KPYM_HUMAN PKM Pyruvate kinase PKM human Yes
Uniprot: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. more..
Dissimilar Functions Manual Curation

Function 1: Pyruvate kinase, glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP. Condition: presence in a tetrameric form.

Function 2: Thyroid hormone-binding protein that regulates the transcriptional responses of the thiroid hormone receptor. Condition: presence in a monomeric form.

Publications (PMID): 15877277

MoonDB Dissimilar Functions
GO ID 1 Function 1 Module ID 1 GO ID 2 Function 2 Module ID 2 Association Probability (PrOnto) Interaction Probability (PrOnto)
GO:0007165 signal transduction 141, 187 GO:0034641 cellular nitrogen compound metabolic process 267 4.22e-144 6.90e-18
GO:0007165 signal transduction 141, 187 GO:0006139 nucleobase-containing compound metabolic process 472 1.10e-111 5.07e-25
GO:0034641 cellular nitrogen compound metabolic process 267 GO:0006928 movement of cell or subcellular component 496 7.55e-40 5.63e-168
GO:0009059 macromolecule biosynthetic process 391 GO:0006928 movement of cell or subcellular component 496 1.49e-13 8.46e-109
GO:0006139 nucleobase-containing compound metabolic process 472 GO:0006928 movement of cell or subcellular component 496 1.95e-31 6.36e-195
Biological Processes
Module ID (MoonGO) GO ID (BP) GO Name
2 GO:0044267 cellular protein metabolic process
2 GO:0010467 gene expression
2 GO:0006139 nucleobase-containing compound metabolic process
2 GO:0060255 regulation of macromolecule metabolic process
2 GO:0007165 signal transduction
29 GO:0060255 regulation of macromolecule metabolic process
29 GO:0007165 signal transduction
42 GO:0031325 positive regulation of cellular metabolic process
42 GO:0010604 positive regulation of macromolecule metabolic process
42 GO:0051173 positive regulation of nitrogen compound metabolic process
42 GO:0006355 regulation of transcription, DNA-templated
42 GO:0007165 signal transduction
45 GO:0071495 cellular response to endogenous stimulus
45 GO:0071310 cellular response to organic substance
45 GO:0032270 positive regulation of cellular protein metabolic process
45 GO:0006468 protein phosphorylation
45 GO:0042325 regulation of phosphorylation
45 GO:0009966 regulation of signal transduction
138 GO:0006464 cellular protein modification process
138 GO:0007165 signal transduction
141 GO:0035556 intracellular signal transduction
141 GO:0006468 protein phosphorylation
141 GO:0060255 regulation of macromolecule metabolic process
187 GO:0007166 cell surface receptor signaling pathway
187 GO:0006464 cellular protein modification process
187 GO:0070887 cellular response to chemical stimulus
187 GO:0035556 intracellular signal transduction
187 GO:0006928 movement of cell or subcellular component
187 GO:0006796 phosphate-containing compound metabolic process
187 GO:0010647 positive regulation of cell communication
187 GO:0048584 positive regulation of response to stimulus
187 GO:0023056 positive regulation of signaling
187 GO:0060255 regulation of macromolecule metabolic process
187 GO:0009966 regulation of signal transduction
202 GO:0044267 cellular protein metabolic process
202 GO:0070887 cellular response to chemical stimulus
202 GO:0060255 regulation of macromolecule metabolic process
202 GO:0007165 signal transduction
267 GO:0044271 cellular nitrogen compound biosynthetic process
267 GO:0010467 gene expression
267 GO:0009059 macromolecule biosynthetic process
267 GO:0090304 nucleic acid metabolic process
267 GO:0019538 protein metabolic process
267 GO:0060255 regulation of macromolecule metabolic process
319 GO:0060255 regulation of macromolecule metabolic process
337 GO:0022607 cellular component assembly
337 GO:0035556 intracellular signal transduction
337 GO:0006468 protein phosphorylation
337 GO:0060255 regulation of macromolecule metabolic process
391 GO:0034645 cellular macromolecule biosynthetic process
391 GO:0090304 nucleic acid metabolic process
391 GO:0034654 nucleobase-containing compound biosynthetic process
391 GO:0060255 regulation of macromolecule metabolic process
391 GO:0019219 regulation of nucleobase-containing compound metabolic process
472 GO:0034654 nucleobase-containing compound biosynthetic process
472 GO:0060255 regulation of macromolecule metabolic process
496 GO:0048870 cell motility
496 GO:0071310 cellular response to organic substance
496 GO:0090304 nucleic acid metabolic process
496 GO:0034654 nucleobase-containing compound biosynthetic process
496 GO:0043085 positive regulation of catalytic activity
496 GO:0031325 positive regulation of cellular metabolic process
496 GO:1902533 positive regulation of intracellular signal transduction
496 GO:0010604 positive regulation of macromolecule metabolic process
496 GO:0051173 positive regulation of nitrogen compound metabolic process
496 GO:0051128 regulation of cellular component organization
496 GO:2000112 regulation of cellular macromolecule biosynthetic process
496 GO:0010468 regulation of gene expression
496 GO:0043549 regulation of kinase activity
496 GO:0019219 regulation of nucleobase-containing compound metabolic process
496 GO:0001932 regulation of protein phosphorylation
496 GO:0009719 response to endogenous stimulus
496 GO:1901700 response to oxygen-containing compound
496 GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
539 GO:0000082 G1/S transition of mitotic cell cycle
539 GO:0034645 cellular macromolecule biosynthetic process
539 GO:0090304 nucleic acid metabolic process
539 GO:0060255 regulation of macromolecule metabolic process
668 GO:0006464 cellular protein modification process
668 GO:0006796 phosphate-containing compound metabolic process
695 GO:0006464 cellular protein modification process
695 GO:0035556 intracellular signal transduction
695 GO:0016310 phosphorylation
695 GO:0060255 regulation of macromolecule metabolic process
702 GO:0007165 signal transduction
724 GO:0016070 RNA metabolic process
724 GO:0035556 intracellular signal transduction
724 GO:0009892 negative regulation of metabolic process
724 GO:0034654 nucleobase-containing compound biosynthetic process
724 GO:0006468 protein phosphorylation
724 GO:2000112 regulation of cellular macromolecule biosynthetic process
724 GO:0032268 regulation of cellular protein metabolic process
724 GO:0010468 regulation of gene expression
724 GO:0019219 regulation of nucleobase-containing compound metabolic process
737 GO:0006464 cellular protein modification process
737 GO:0009059 macromolecule biosynthetic process
737 GO:0006139 nucleobase-containing compound metabolic process
737 GO:0031325 positive regulation of cellular metabolic process
737 GO:0010604 positive regulation of macromolecule metabolic process
737 GO:0051173 positive regulation of nitrogen compound metabolic process
737 GO:0032268 regulation of cellular protein metabolic process
737 GO:0007165 signal transduction
831 GO:0007166 cell surface receptor signaling pathway
831 GO:0044271 cellular nitrogen compound biosynthetic process
831 GO:0006139 nucleobase-containing compound metabolic process
831 GO:0006796 phosphate-containing compound metabolic process
831 GO:0010646 regulation of cell communication
831 GO:0060255 regulation of macromolecule metabolic process
831 GO:0023051 regulation of signaling
833 GO:0034645 cellular macromolecule biosynthetic process
833 GO:0006464 cellular protein modification process
833 GO:0006796 phosphate-containing compound metabolic process
833 GO:0060255 regulation of macromolecule metabolic process
Cellular Components
Module ID (MoonGO) GO ID (CC) GO Name
2 GO:0005829 cytosol
2 GO:0005634 nucleus
29 GO:0005634 nucleus
42 GO:0005829 cytosol
42 GO:0005654 nucleoplasm
45 GO:0005829 cytosol
45 GO:0031981 nuclear lumen
45 GO:0005886 plasma membrane
138 GO:0005634 nucleus
141 GO:0005634 nucleus
187 GO:0005829 cytosol
187 GO:0005886 plasma membrane
202 GO:0005829 cytosol
202 GO:0005886 plasma membrane
267 GO:0005829 cytosol
267 GO:0070013 intracellular organelle lumen
267 GO:0005634 nucleus
319 GO:0005829 cytosol
319 GO:0005634 nucleus
337 GO:0005829 cytosol
337 GO:0043232 intracellular non-membrane-bounded organelle
337 GO:0005634 nucleus
391 GO:0070013 intracellular organelle lumen
391 GO:0005634 nucleus
472 GO:0043232 intracellular non-membrane-bounded organelle
472 GO:0005634 nucleus
496 GO:0005829 cytosol
496 GO:0005634 nucleus
496 GO:0005886 plasma membrane
539 GO:0043232 intracellular non-membrane-bounded organelle
539 GO:0005654 nucleoplasm
695 GO:0005829 cytosol
695 GO:0005634 nucleus
702 GO:0005829 cytosol
724 GO:0005829 cytosol
724 GO:0070013 intracellular organelle lumen
724 GO:0005634 nucleus
737 GO:0005829 cytosol
737 GO:0043232 intracellular non-membrane-bounded organelle
737 GO:0005634 nucleus
831 GO:0042995 cell projection
831 GO:0005829 cytosol
831 GO:0005634 nucleus
831 GO:0005886 plasma membrane
833 GO:0005829 cytosol
GO ID (BP) GO Name Evidence Code (GO EC)
GO:0001666 response to hypoxia IEA
GO:0001889 liver development IEA
GO:0006754 ATP biosynthetic process IEA
GO:0007584 response to nutrient IEA
GO:0009629 response to gravity IEA
GO:0012501 programmed cell death IDA
GO:0014870 response to muscle inactivity IEA
GO:0031100 animal organ regeneration IEA
GO:0032869 cellular response to insulin stimulus IBA
GO:0042866 pyruvate biosynthetic process IEA
GO:0043312 neutrophil degranulation TAS
GO:0043403 skeletal muscle tissue regeneration IEA
GO:0051289 protein homotetramerization IEA
GO:0061621 canonical glycolysis TAS
GO ID (CC) GO Name Evidence Code (GO EC)
GO:0005576 extracellular region TAS
GO:0005634 nucleus IDA
GO:0005737 cytoplasm IDA
GO:0005739 mitochondrion IDA
GO:0005829 cytosol TAS
GO:0005929 cilium IEA
GO:0031012 extracellular matrix IDA
GO:0031982 vesicle IDA
GO:0034774 secretory granule lumen TAS
GO:0043209 myelin sheath IEA
GO:0070062 extracellular exosome IDA
GO:1902912 pyruvate kinase complex IEA
GO:1903561 extracellular vesicle IDA
GO:1904813 ficolin-1-rich granule lumen TAS
GO ID 1 Component 1 GO ID 2 Component 2 Association Probability (PrOnto) Interaction Probability (PrOnto)
GO:0005576 extracellular region GO:0005634 nucleus 4.48e-91 1.28e-107
GO:0005634 nucleus GO:0031012 extracellular matrix 1.58e-13 4.18e-03
GO:0005634 nucleus GO:0031982 vesicle 1.94e-54 3.27e-93
GO:0005634 nucleus GO:0070062 extracellular exosome 6.30e-13 4.54e-26
GO:0005634 nucleus GO:1903561 extracellular vesicle 1.85e-13 4.00e-27
GO:0005829 cytosol GO:0031012 extracellular matrix 4.46e-06 5.18e-08
Interactor Also a MoonDB EMF protein
PAK5_HUMAN No
T22D4_HUMAN No
HMBX1_HUMAN No
GRB7_HUMAN No
EXOS5_HUMAN No
BLK_HUMAN No
SMN_HUMAN No
RHG07_HUMAN No
PIN1_HUMAN No
AAPK2_HUMAN No
LNX1_HUMAN Yes: Q8TBB1 (MoonDB)
NOE2_HUMAN No
PCM1_HUMAN Yes: Q15154 (MoonDB)
HD_HUMAN Yes: P42858 (MoonDB)
KCC2B_HUMAN No
EGFR_HUMAN Yes: P00533 (MoonDB)
HSF1_HUMAN Yes: Q00613 (MoonDB)
RXRA_HUMAN Yes: P19793 (MoonDB)
STK25_HUMAN No
NXT2_HUMAN No
NEK6_HUMAN No
RAF1_HUMAN Yes: P04049 (MoonDB)
ERBB2_HUMAN Yes: P04626 (MoonDB)
R3HD2_HUMAN No
H31_HUMAN No
AKT1_HUMAN No
NAGK_HUMAN No
TYRO3_HUMAN No
KITH_HUMAN No
SOX5_HUMAN No
DCLK2_HUMAN No
RPP14_HUMAN No
FAK1_HUMAN No
SOCS3_HUMAN No
PDPK1_HUMAN Yes: O15530 (MoonDB)
KS6A5_HUMAN No
TF65_HUMAN No
KBTB7_HUMAN No
ABL1_HUMAN Yes: P00519 (MoonDB)
H33_HUMAN No
H32_HUMAN No
THA_HUMAN No
CDK4_HUMAN No
CDN1A_HUMAN Yes: P38936 (MoonDB)
MET_HUMAN No
KIT_HUMAN No
KSYK_HUMAN Yes: P43405 (MoonDB)
MDM2_HUMAN Yes: Q00987 (MoonDB)
BIN1_HUMAN Yes: O00499 (MoonDB)
ABL2_HUMAN Yes: P42684 (MoonDB)
ADDA_HUMAN No
AKT3_HUMAN No
ALDOC_HUMAN No
ALK_HUMAN No
ANXA7_HUMAN No
AP180_HUMAN No
ARAF_HUMAN No
BEAN1_HUMAN No
BMI1_HUMAN No
BMX_HUMAN No
BRSK1_HUMAN No
CARTF_HUMAN No
CASS4_HUMAN No
CASZ1_HUMAN No
CBARP_HUMAN No
CCDC6_HUMAN No
CHK1_HUMAN No
CHK2_HUMAN No
COE4_HUMAN No
CP4FC_HUMAN No
CQ082_HUMAN No
CSF1R_HUMAN No
CYH1_HUMAN No
DAPLE_HUMAN No
DEMA_HUMAN No
DIXC1_HUMAN No
DPOE2_HUMAN No
DYR1B_HUMAN No
DYRK3_HUMAN No
E400N_HUMAN No
ENOB_HUMAN No
EPHA1_HUMAN No
EPHA2_HUMAN No
EPHA5_HUMAN No
EPHA8_HUMAN No
EPHB3_HUMAN No
EPHB4_HUMAN No
EPM2A_HUMAN No
F122A_HUMAN No
FER_HUMAN No
FGFR1_HUMAN No
FGFR2_HUMAN No
FGFR3_HUMAN No
FGFR4_HUMAN No
FSD1_HUMAN No
GSK3A_HUMAN No
HERC1_HUMAN No
HIPK1_HUMAN No
IF4G3_HUMAN No
IKKA_HUMAN No
IKKB_HUMAN No
IKKE_HUMAN No
IL1B_HUMAN No
JAK2_HUMAN No
JAK3_HUMAN No
KANK4_HUMAN No
KGP2_HUMAN No
KI26A_HUMAN No
KKCC2_HUMAN No
KPCD1_HUMAN No
KPCD3_HUMAN No
KPCE_HUMAN No
KPCT_HUMAN No
KS6A2_HUMAN No
KS6A3_HUMAN No
LMO7_HUMAN No
LTK_HUMAN No
M3K11_HUMAN No
M3K2_HUMAN No
M3K3_HUMAN No
M4K2_HUMAN No
MAPK2_HUMAN No
MAPK3_HUMAN No
MAPK5_HUMAN No
MARK2_HUMAN No
MEF2A_HUMAN No
MEF2D_HUMAN No
MERTK_HUMAN No
MK03_HUMAN Yes: P27361 (MoonDB)
MK12_HUMAN No
MK13_HUMAN No
MP2K1_HUMAN No
MP2K2_HUMAN No
MPIP1_HUMAN No
MSMO1_HUMAN No
MYOC_HUMAN No
NEK1_HUMAN No
NFIC_HUMAN No
NFYA_HUMAN No
NLK_HUMAN No
NR1D1_HUMAN No
NTRK2_HUMAN No
NTRK3_HUMAN No
NUP53_HUMAN No
PAK2_HUMAN No
PAK6_HUMAN No
PAPOA_HUMAN No
PAX8_HUMAN No
PCIF1_HUMAN No
PEPD_HUMAN No
PGFRA_HUMAN No
PHAR4_HUMAN No
PRSR2_HUMAN No
RAP1B_HUMAN No
RCC1_HUMAN No
RET_HUMAN No
RON_HUMAN No
ROS1_HUMAN No
SDCG3_HUMAN No
SHBG_HUMAN Yes: P04278 (MoonDB)
STK33_HUMAN No
STK4_HUMAN No
SWET1_HUMAN No
TAOK2_HUMAN No
TBK1_HUMAN No
TERF1_HUMAN No
TFF2_HUMAN No
TIE2_HUMAN No
TINF2_HUMAN Yes: Q9BSI4 (MoonDB)
TITIN_HUMAN Yes: Q8WZ42 (MoonDB)
TOX2_HUMAN No
TRI63_HUMAN No
TSSK2_HUMAN No
VGFR1_HUMAN No
VGFR2_HUMAN No
VGFR3_HUMAN No
WAC_HUMAN No
WDR62_HUMAN No
WNK2_HUMAN No
ZFAN5_HUMAN No
ZXDC_HUMAN No
PMID Article Title
1854723 An in vitro novel mechanism of regulating the activity of pyruvate kinase M2 by thyroid hormone and fructose 1, 6-bisphosphate.
2040271 Isolation and characterization of the human pyruvate kinase M gene.
2813362 Cytosolic thyroid hormone-binding protein is a monomer of pyruvate kinase.
2854097 Human M2-type pyruvate kinase: cDNA cloning, chromosomal assignment and expression in hepatoma.
9466265 Using the yeast two-hybrid system to identify human epithelial cell proteins that bind gonococcal Opa proteins: intracellular gonococci bind pyruvate kinase via their Opa proteins and require host pyruvate for growth.
12650930 Interaction between HERC1 and M2-type pyruvate kinase.
12665801 Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.
14702039 Complete sequencing and characterization of 21,243 full-length human cDNAs.
15489334 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
15592455 Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.
15996096 Structural basis for tumor pyruvate kinase M2 allosteric regulation and catalysis.
16139798 Proteomic identification of proteins conjugated to ISG15 in mouse and human cells.
16572171 Analysis of the DNA sequence and duplication history of human chromosome 15.
16964243 A probability-based approach for high-throughput protein phosphorylation analysis and site localization.
17081983 Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.
17308100 Nuclear translocation of the tumor marker pyruvate kinase M2 induces programmed cell death.
18088087 Phosphoproteome of resting human platelets.
18191611 Pyruvate kinase isozyme type M2 (PKM2) interacts and cooperates with Oct-4 in regulating transcription.
18298799 Modulation of M2-type pyruvate kinase activity by the cytoplasmic PML tumor suppressor protein.
18337815 Pyruvate kinase M2 is a phosphotyrosine-binding protein.
18669648 A quantitative atlas of mitotic phosphorylation.
18691976 Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.
19413330 Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.
19608861 Lysine acetylation targets protein complexes and co-regulates major cellular functions.
19690332 Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.
20068231 Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.
21269460 Initial characterization of the human central proteome.
21406692 System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.
21483450 The oxygen sensor PHD3 limits glycolysis under hypoxia via direct binding to pyruvate kinase.
21620138 Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1.
21700219 Acetylation targets the M2 isoform of pyruvate kinase for degradation through chaperone-mediated autophagy and promotes tumor growth.
23064226 Serine is a natural ligand and allosteric activator of pyruvate kinase M2.
23186163 Toward a comprehensive characterization of a human cancer cell phosphoproteome.
23530218 M2 pyruvate kinase provides a mechanism for nutrient sensing and regulation of cell proliferation.
24129315 Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.
24275569 An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.
25114211 Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli.
25263439 TRIM35 Interacts with pyruvate kinase isoform M2 to suppress the Warburg effect and tumorigenicity in hepatocellular carcinoma.
25944712 N-terminome analysis of the human mitochondrial proteome.
28112733 Site-specific mapping of the human SUMO proteome reveals co-modification with phosphorylation.
No results found.
Domain Name Domain ID Source
Pyr_Knase IPR001697 InterPro
Pyrv_Knase-like_insert_dom_sf IPR011037 InterPro
Pyrv_Knase_brl IPR015793 InterPro
Pyrv_Knase_C IPR015795 InterPro
Pyrv_Knase_insert_dom_sf IPR015806 InterPro
Pyrv/PenolPyrv_Kinase-like_dom IPR015813 InterPro
Pyrv_Knase_AS IPR018209 InterPro
Pyrv_Knase_C_sf IPR036918 InterPro
PK PF00224 Pfam
PK_C PF02887 Pfam
PYRUVTKNASE PR01050 PRINTS
PYRUVATE_KINASE PS00110 PROSITE
PTHR11817 PTHR11817 PANTHER
S30038 S30038 PIR
S64635 S64635 PIR
SSF50800 SSF50800 SUPFAM
SSF51621 SSF51621 SUPFAM
SSF52935 SSF52935 SUPFAM
pyruv_kin TIGR01064 TIGRFAMs
Pyruvate_Kinase cd00288 CDD