Protein: O15530

UniprotKB AC UniprotKB ID Gene name Full name Species Curated set
O15530 (Uniprot) PDPK1_HUMAN PDPK1 3-phosphoinositide-dependent protein kinase 1 human No
Uniprot: Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptional activity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controls proliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca(2+) entry and Ca(2+)-activated K(+) channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses. Provides negative feedback inhibition to toll-like receptor-mediated NF-kappa-B activation in macrophages. Isoform 3 is catalytically inactive. more..
GO ID 1 Function 1 Module ID 1 GO ID 2 Function 2 Module ID 2 Association Probability (PrOnto) Interaction Probability (PrOnto)
GO:0006725 cellular aromatic compound metabolic process 222 GO:0007165 signal transduction 355 5.44e-126 5.27e-27
Module ID (MoonGO) GO ID (BP) GO Name
2 GO:0044267 cellular protein metabolic process
2 GO:0010467 gene expression
2 GO:0006139 nucleobase-containing compound metabolic process
2 GO:0060255 regulation of macromolecule metabolic process
2 GO:0007165 signal transduction
31 GO:0034645 cellular macromolecule biosynthetic process
31 GO:0044271 cellular nitrogen compound biosynthetic process
31 GO:0090304 nucleic acid metabolic process
31 GO:0010468 regulation of gene expression
173 GO:0034645 cellular macromolecule biosynthetic process
173 GO:0006464 cellular protein modification process
173 GO:0010467 gene expression
173 GO:0035556 intracellular signal transduction
173 GO:0090304 nucleic acid metabolic process
173 GO:0016310 phosphorylation
173 GO:0031325 positive regulation of cellular metabolic process
173 GO:0010604 positive regulation of macromolecule metabolic process
173 GO:0051173 positive regulation of nitrogen compound metabolic process
173 GO:0031326 regulation of cellular biosynthetic process
173 GO:0019219 regulation of nucleobase-containing compound metabolic process
222 GO:0006139 nucleobase-containing compound metabolic process
222 GO:0060255 regulation of macromolecule metabolic process
222 GO:0007165 signal transduction
355 GO:0007166 cell surface receptor signaling pathway
355 GO:0006464 cellular protein modification process
355 GO:0035556 intracellular signal transduction
355 GO:0016310 phosphorylation
355 GO:0010647 positive regulation of cell communication
355 GO:0048584 positive regulation of response to stimulus
355 GO:0023056 positive regulation of signaling
355 GO:0060255 regulation of macromolecule metabolic process
355 GO:0009966 regulation of signal transduction
Module ID (MoonGO) GO ID (CC) GO Name
2 GO:0005829 cytosol
2 GO:0005634 nucleus
31 GO:0005634 nucleus
173 GO:0005654 nucleoplasm
222 GO:0070013 intracellular organelle lumen
222 GO:0044428 nuclear part
355 GO:0044459 plasma membrane part
GO ID (BP) GO Name Evidence Code (GO EC)
GO:0002223 stimulatory C-type lectin receptor signaling pathway TAS
GO:0003323 type B pancreatic cell development IEA
GO:0006351 transcription, DNA-templated IEA
GO:0006355 regulation of transcription, DNA-templated IEA
GO:0006468 protein phosphorylation IDA
GO:0006469 negative regulation of protein kinase activity IDA
GO:0006972 hyperosmotic response IEA
GO:0007173 epidermal growth factor receptor signaling pathway IMP
GO:0010518 positive regulation of phospholipase activity IMP
GO:0010594 regulation of endothelial cell migration IEA
GO:0010667 negative regulation of cardiac muscle cell apoptotic process IEA
GO:0016477 cell migration IMP
GO:0018105 peptidyl-serine phosphorylation IBA
GO:0018107 peptidyl-threonine phosphorylation IDA
GO:0019722 calcium-mediated signaling IMP
GO:0030036 actin cytoskeleton organization TAS
GO:0030168 platelet activation TAS
GO:0030512 negative regulation of transforming growth factor beta receptor signaling pathway IDA
GO:0031295 T cell costimulation TAS
GO:0032148 activation of protein kinase B activity TAS
GO:0032869 cellular response to insulin stimulus IMP
GO:0034122 negative regulation of toll-like receptor signaling pathway IEA
GO:0035556 intracellular signal transduction IDA
GO:0038095 Fc-epsilon receptor signaling pathway TAS
GO:0043122 regulation of I-kappaB kinase/NF-kappaB signaling IMP
GO:0043304 regulation of mast cell degranulation IEA
GO:0043524 negative regulation of neuron apoptotic process IEA
GO:0046777 protein autophosphorylation TAS
GO:0048041 focal adhesion assembly IEA
GO:0050852 T cell receptor signaling pathway TAS
GO:0051281 positive regulation of release of sequestered calcium ion into cytosol IDA
GO:0071364 cellular response to epidermal growth factor stimulus IMP
GO:0097191 extrinsic apoptotic signaling pathway IMP
GO:1903078 positive regulation of protein localization to plasma membrane IMP
GO:1990416 cellular response to brain-derived neurotrophic factor stimulus IEA
GO ID (CC) GO Name Evidence Code (GO EC)
GO:0005654 nucleoplasm TAS
GO:0005737 cytoplasm IDA
GO:0005829 cytosol TAS
GO:0005886 plasma membrane TAS
GO:0005925 focal adhesion IEA
GO:0014069 postsynaptic density IEA
GO:0031410 cytoplasmic vesicle IEA
GO:0042995 cell projection IDA
GO:0043204 perikaryon IEA
GO ID 1 Component 1 GO ID 2 Component 2 Association Probability (PrOnto) Interaction Probability (PrOnto)
GO:0005654 nucleoplasm GO:0005886 plasma membrane 9.14e-199 6.17e-196
GO:0005654 nucleoplasm GO:0031410 cytoplasmic vesicle 2.02e-39 4.57e-107
GO:0005654 nucleoplasm GO:0042995 cell projection 1.49e-18 8.51e-113
PMID Article Title
9094314 Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase B alpha.
9368760 3-phosphoinositide-dependent protein kinase-1 (PDK1): structural and functional homology with the Drosophila DSTPK61 kinase.
9445476 Phosphorylation and activation of p70s6k by PDK1.
9445477 Protein kinase B kinases that mediate phosphatidylinositol 3,4,5-trisphosphate-dependent activation of protein kinase B.
9637919 Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B.
9707564 Phosphorylation and activation of cAMP-dependent protein kinase by phosphoinositide-dependent protein kinase.
9768361 Regulation of protein kinase C zeta by PI 3-kinase and PDK-1.
10226025 PDK1 acquires PDK2 activity in the presence of a synthetic peptide derived from the carboxyl terminus of PRK2.
10364160 A PDK1 homolog is necessary and sufficient to transduce AGE-1 PI3 kinase signals that regulate diapause in Caenorhabditis elegans.
10455013 Phosphorylation of Ser-241 is essential for the activity of 3-phosphoinositide-dependent protein kinase-1: identification of five sites of phosphorylation in vivo.
10480933 90-kDa ribosomal S6 kinase is phosphorylated and activated by 3-phosphoinositide-dependent protein kinase-1.
10995762 p21-activated kinase (PAK1) is phosphorylated and activated by 3-phosphoinositide-dependent kinase-1 (PDK1).
11481331 Identification of tyrosine phosphorylation sites on 3-phosphoinositide-dependent protein kinase-1 (PDK1) and their role in regulating kinase activity.
12167717 Multiple phosphoinositide 3-kinase-dependent steps in activation of protein kinase B.
12169624 High resolution crystal structure of the human PDK1 catalytic domain defines the regulatory phosphopeptide docking site.
12177059 Regulation of kinase activity of 3-phosphoinositide-dependent protein kinase-1 by binding to 14-3-3.
14585963 Pyk2- and Src-dependent tyrosine phosphorylation of PDK1 regulates focal adhesions.
14604990 Peroxisomal targeting as a tool for assaying protein-protein interactions in the living cell: cytokine-independent survival kinase (CISK) binds PDK-1 in vivo in a phosphorylation-dependent manner.
15209375 PDK1, the master regulator of AGC kinase signal transduction.
15210700 The adaptor protein Grb14 regulates the localization of 3-phosphoinositide-dependent kinase-1.
15489334 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
15616553 The sequence and analysis of duplication-rich human chromosome 16.
15741170 Role of T-loop phosphorylation in PDK1 activation, stability, and substrate binding.
15743829 Phosphoinositide-dependent phosphorylation of PDK1 regulates nuclear translocation.
15772071 Novel small molecule inhibitors of 3-phosphoinositide-dependent kinase-1.
16207722 3-Phosphoinositide-dependent protein kinase-1-mediated IkappaB kinase beta (IkkB) phosphorylation activates NF-kappaB signaling.
16251192 Regulation of transforming growth factor-beta signaling and PDK1 kinase activity by physical interaction between PDK1 and serine-threonine kinase receptor-associated protein.
16314505 Tyrosine phosphorylation of phosphoinositide-dependent kinase 1 by the insulin receptor is necessary for insulin metabolic signaling.
16780920 Role of the PH domain in regulating in vitro autophosphorylation events required for reconstitution of PDK1 catalytic activity.
17327236 3-Phosphoinositide-dependent PDK1 negatively regulates transforming growth factor-beta-induced signaling in a kinase-dependent manner through physical interaction with Smad proteins.
17371830 Essential role of PDK1 in regulating endothelial cell migration.
18024423 Regulation of 3-phosphoinositide-dependent protein kinase-1 (PDK1) by Src involves tyrosine phosphorylation of PDK1 and Src homology 2 domain binding.
18616680 TUSC4/NPRL2, a novel PDK1-interacting protein, inhibits PDK1 tyrosine phosphorylation and its downstream signaling.
18802401 Dissecting the role of the 3-phosphoinositide-dependent protein kinase-1 (PDK1) signalling pathways.
18835241 The C-terminus of PRK2/PKNgamma is required for optimal activation by RhoA in a GTP-dependent manner.
19276999 IGF-I regulated phosphorylation and translocation of PDK-1 in neurons.
19591923 The nuclear localization of 3'-phosphoinositide-dependent kinase-1 is dependent on its association with the protein tyrosine phosphatase SHP-1.
19690332 Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.
20978239 Regulation of 3-phosphoinositide-dependent protein kinase 1 activity by homodimerization in live cells.
22347420 Ubiquitin-specific protease 4 inhibits mono-ubiquitination of the master growth factor signaling kinase PDK1.
22544756 PDK1 phosphorylation at Thr354 by murine protein serine/threonine kinase 38 contributes to the negative regulation of PDK1 activity.
22999883 Substrate-selective inhibition of protein kinase PDK1 by small compounds that bind to the PIF-pocket allosteric docking site.
24275569 An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.
No results found.
Domain Name Domain ID Source
Prot_kinase_dom IPR000719 InterPro
Ser/Thr_kinase_AS IPR008271 InterPro
Kinase-like_dom_sf IPR011009 InterPro
PH-like_dom_sf IPR011993 InterPro
Protein_kinase_ATP_BS IPR017441 InterPro
PDK1-typ_PH IPR033931 InterPro
Pkinase PF00069 Pfam
PH_3 PF14593 Pfam
SSF50729 SSF50729 SUPFAM
SSF56112 SSF56112 SUPFAM
PH_PDK1 cd01262 CDD