Protein: Q99816

UniprotKB AC UniprotKB ID Gene name Full name Species Curated set
Q99816 (Uniprot) TS101_HUMAN TSG101 Tumor susceptibility gene 101 protein human Yes
Uniprot: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). It may also play a role in the extracellular release of microvesicles that differ from the exosomes (PubMed:22315426). more..
Dissimilar Functions Manual Curation

Function 1: Transport, part of the ESCRT complex (endocytosis). Condition: mainly cytoplasmic, membrane-associated when active and soluble when inactive.

Function 2: Transcriptional repressor protein, decreasing GR-dependent transcriptional activation by binding and repressing AF-1. Condition: nuclear localisation (cell cycle dependant, nuclear localisation increased through binding of nuclear proteins AATF, Daxx or RTA).

Publications (PMID): 10508170, 9588212, 9465061, 17182691

MoonDB Dissimilar Functions
GO ID 1 Function 1 Module ID 1 GO ID 2 Function 2 Module ID 2 Association Probability (PrOnto) Interaction Probability (PrOnto)
GO:0009056 catabolic process 48, 125 GO:0009059 macromolecule biosynthetic process 672 6.37e-04 1.88e-40
GO:0007165 signal transduction 255 GO:0006139 nucleobase-containing compound metabolic process 713 1.10e-111 5.07e-25
Biological Processes
Module ID (MoonGO) GO ID (BP) GO Name
48 GO:0044248 cellular catabolic process
48 GO:0070647 protein modification by small protein conjugation or removal
48 GO:0006508 proteolysis
48 GO:0060255 regulation of macromolecule metabolic process
48 GO:0007165 signal transduction
67 GO:0060255 regulation of macromolecule metabolic process
116 GO:0019538 protein metabolic process
116 GO:0060255 regulation of macromolecule metabolic process
125 GO:0044248 cellular catabolic process
125 GO:0016567 protein ubiquitination
125 GO:0060255 regulation of macromolecule metabolic process
134 GO:0044267 cellular protein metabolic process
134 GO:0060255 regulation of macromolecule metabolic process
134 GO:0007165 signal transduction
166 GO:0044267 cellular protein metabolic process
166 GO:0006139 nucleobase-containing compound metabolic process
166 GO:0060255 regulation of macromolecule metabolic process
166 GO:0007165 signal transduction
181 GO:0044267 cellular protein metabolic process
181 GO:0010467 gene expression
181 GO:0009059 macromolecule biosynthetic process
181 GO:0090304 nucleic acid metabolic process
181 GO:0060255 regulation of macromolecule metabolic process
181 GO:0007165 signal transduction
198 GO:0060255 regulation of macromolecule metabolic process
198 GO:0007165 signal transduction
240 GO:0019538 protein metabolic process
240 GO:0007165 signal transduction
255 GO:0008283 cell proliferation
255 GO:0007166 cell surface receptor signaling pathway
255 GO:0006464 cellular protein modification process
255 GO:0060429 epithelium development
255 GO:0006139 nucleobase-containing compound metabolic process
255 GO:0006796 phosphate-containing compound metabolic process
255 GO:0006508 proteolysis
351 GO:0022402 cell cycle process
351 GO:0034645 cellular macromolecule biosynthetic process
351 GO:0044267 cellular protein metabolic process
351 GO:0090304 nucleic acid metabolic process
403 GO:0060255 regulation of macromolecule metabolic process
420 GO:0006464 cellular protein modification process
507 GO:0010467 gene expression
507 GO:0006139 nucleobase-containing compound metabolic process
540 GO:0022607 cellular component assembly
552 GO:0044267 cellular protein metabolic process
672 GO:0034645 cellular macromolecule biosynthetic process
672 GO:0006139 nucleobase-containing compound metabolic process
672 GO:1901362 organic cyclic compound biosynthetic process
672 GO:0009889 regulation of biosynthetic process
672 GO:0010468 regulation of gene expression
677 GO:0022607 cellular component assembly
713 GO:0090304 nucleic acid metabolic process
713 GO:0070647 protein modification by small protein conjugation or removal
713 GO:0007165 signal transduction
Cellular Components
Module ID (MoonGO) GO ID (CC) GO Name
48 GO:0031410 cytoplasmic vesicle
48 GO:0012505 endomembrane system
48 GO:0005634 nucleus
48 GO:0005886 plasma membrane
67 GO:0005634 nucleus
116 GO:0005829 cytosol
116 GO:0070013 intracellular organelle lumen
116 GO:0005634 nucleus
125 GO:0005829 cytosol
125 GO:0005634 nucleus
134 GO:0005829 cytosol
134 GO:0005634 nucleus
140 GO:0043232 intracellular non-membrane-bounded organelle
166 GO:0005829 cytosol
166 GO:0070013 intracellular organelle lumen
166 GO:0044428 nuclear part
166 GO:0005886 plasma membrane
181 GO:0005829 cytosol
181 GO:0005654 nucleoplasm
198 GO:0005829 cytosol
240 GO:0005615 extracellular space
255 GO:0012505 endomembrane system
255 GO:0016021 integral component of membrane
255 GO:0005886 plasma membrane
351 GO:0043232 intracellular non-membrane-bounded organelle
351 GO:0005654 nucleoplasm
403 GO:0005634 nucleus
420 GO:0005829 cytosol
420 GO:0005634 nucleus
507 GO:0044428 nuclear part
540 GO:0005829 cytosol
540 GO:0043232 intracellular non-membrane-bounded organelle
540 GO:0005634 nucleus
552 GO:0005634 nucleus
628 GO:0005829 cytosol
628 GO:0070062 extracellular exosome
628 GO:0005634 nucleus
672 GO:0031981 nuclear lumen
677 GO:0005813 centrosome
677 GO:0005829 cytosol
677 GO:0005634 nucleus
713 GO:0005829 cytosol
713 GO:0005634 nucleus
GO ID (BP) GO Name Evidence Code (GO EC)
GO:0001558 regulation of cell growth IEA
GO:0006513 protein monoubiquitination IEA
GO:0006858 extracellular transport IMP
GO:0007050 cell cycle arrest IEA
GO:0007175 negative regulation of epidermal growth factor-activated receptor activity IMP
GO:0008285 negative regulation of cell proliferation IEA
GO:0008333 endosome to lysosome transport IEA
GO:0015031 protein transport IEA
GO:0016197 endosomal transport TAS
GO:0016236 macroautophagy TAS
GO:0019058 viral life cycle TAS
GO:0030216 keratinocyte differentiation IEA
GO:0036258 multivesicular body assembly TAS
GO:0039702 viral budding via host ESCRT complex TAS
GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway IMP
GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway IMP
GO:0043405 regulation of MAP kinase activity IMP
GO:0045892 negative regulation of transcription, DNA-templated IEA
GO:0046755 viral budding IMP
GO:0048524 positive regulation of viral process IMP
GO:0051301 cell division IEA
GO:0075733 intracellular transport of virus TAS
GO:0097352 autophagosome maturation TAS
GO:1902186 regulation of viral release from host cell IMP
GO:1902188 positive regulation of viral release from host cell IMP
GO:1903543 positive regulation of exosomal secretion IMP
GO:1903551 regulation of extracellular exosome assembly IMP
GO:1903772 regulation of viral budding via host ESCRT complex IMP
GO:1903774 positive regulation of viral budding via host ESCRT complex IMP
GO:1990182 exosomal secretion IEA
GO:2000397 positive regulation of ubiquitin-dependent endocytosis IEA
GO ID (CC) GO Name Evidence Code (GO EC)
GO:0000813 ESCRT I complex IDA
GO:0005730 nucleolus IDA
GO:0005737 cytoplasm IDA
GO:0005768 endosome IDA
GO:0005769 early endosome IDA
GO:0005770 late endosome IMP
GO:0005771 multivesicular body TAS
GO:0005815 microtubule organizing center IEA
GO:0005829 cytosol IDA
GO:0005886 plasma membrane IDA
GO:0010008 endosome membrane TAS
GO:0031901 early endosome membrane IEA
GO:0031902 late endosome membrane IEA
GO:0043657 host cell IEA
GO:0070062 extracellular exosome IDA
GO:0090543 Flemming body IEA
GO ID 1 Component 1 GO ID 2 Component 2 Association Probability (PrOnto) Interaction Probability (PrOnto)
GO:0005730 nucleolus GO:0005886 plasma membrane 7.70e-47 3.09e-07
GO:0005815 microtubule organizing center GO:0005886 plasma membrane 3.20e-06 1.55e-21
Interactor Also a MoonDB EMF protein
RBPMS_HUMAN Yes: Q93062 (MoonDB)
TFP11_HUMAN No
KRT34_HUMAN No
K2C75_HUMAN No
K1C13_HUMAN No
K1C15_HUMAN No
GLYR1_HUMAN No
KRT35_HUMAN No
PKNX2_HUMAN No
KIFC3_HUMAN No
TRI54_HUMAN No
VP37C_HUMAN Yes: A5D8V6 (MoonDB)
HAUS1_HUMAN No
BIRC2_HUMAN No
USBP1_HUMAN No
RNF41_HUMAN No
K1H1_HUMAN No
TNIP1_HUMAN No
CCHCR_HUMAN No
ABCB6_HUMAN No
ACAD8_HUMAN No
ADRB2_HUMAN Yes: P07550 (MoonDB)
AJUBA_HUMAN No
AKAP9_HUMAN No
ALG2_HUMAN No
AMPL_HUMAN No
ANDR_HUMAN Yes: P10275 (MoonDB)
ARRD1_HUMAN No
ASPP1_HUMAN No
AT5F1_HUMAN No
BAG4_HUMAN No
BCAS3_HUMAN No
BCR_HUMAN No
CAGE1_HUMAN No
CBY1_HUMAN No
CC88B_HUMAN No
CDN1A_HUMAN Yes: P38936 (MoonDB)
CDS2_HUMAN No
CEP55_HUMAN No
CEP63_HUMAN No
COPE_HUMAN No
CSN6_HUMAN No
DCTN2_HUMAN No
DMAP1_HUMAN No
DPOD2_HUMAN No
DZIP3_HUMAN No
ECHP_HUMAN No
ENOA_HUMAN Yes: P06733 (MoonDB)
F110A_HUMAN No
FAM9B_HUMAN Yes: Q8IZU0 (MoonDB)
FPPS_HUMAN No
FRIL_HUMAN No
GCR_HUMAN No
GGA1_HUMAN No
GGA3_HUMAN No
H0Y7E6_HUMAN No
HGS_HUMAN No
HNRL1_HUMAN No
HSH2D_HUMAN No
INCA1_HUMAN No
IQGA1_HUMAN No
ITSN1_HUMAN No
JUN_HUMAN No
K1328_HUMAN No
K1C16_HUMAN No
K1C18_HUMAN No
KIF5A_HUMAN No
KLK5_HUMAN No
KLK6_HUMAN No
LENG8_HUMAN No
LIPB2_HUMAN No
LNX1_HUMAN Yes: Q8TBB1 (MoonDB)
LRSM1_HUMAN Yes: Q6UWE0 (MoonDB)
MGRN1_HUMAN No
MIC60_HUMAN No
MKRN3_HUMAN Yes: Q13064 (MoonDB)
MTA3_HUMAN No
NDUAD_HUMAN No
NELFD_HUMAN No
NGAL_HUMAN Yes: P80188 (MoonDB)
NT5D2_HUMAN No
ORC1_HUMAN No
PDC6I_HUMAN Yes: Q8WUM4 (MoonDB)
PDLI7_HUMAN No
PIGQ_HUMAN No
PTN23_HUMAN No
RAD18_HUMAN No
RFIP3_HUMAN No
RFIP4_HUMAN No
RIR2_HUMAN No
RN111_HUMAN Yes: Q6ZNA4 (MoonDB)
RN126_HUMAN No
ROCK1_HUMAN No
RSLAB_HUMAN No
SCNNA_HUMAN No
SCRIB_HUMAN Yes: Q14160 (MoonDB)
SG3A1_HUMAN No
SH23A_HUMAN No
SMRC2_HUMAN No
SNF8_HUMAN No
STK11_HUMAN No
SYCE1_HUMAN No
TAXB1_HUMAN Yes: Q86VP1 (MoonDB)
TFF1_HUMAN No
THB_HUMAN No
THOC7_HUMAN No
THRSP_HUMAN No
TM1L1_HUMAN Yes: O75674 (MoonDB)
TNNT3_HUMAN No
TRAIP_HUMAN No
TRI23_HUMAN No
TRI35_HUMAN No
TRI46_HUMAN No
TRI74_HUMAN No
TRIMM_HUMAN No
TSP50_HUMAN No
UBB_HUMAN Yes: P0CG47 (MoonDB)
UBC_HUMAN Yes: P0CG48 (MoonDB)
VP37A_HUMAN No
VP37B_HUMAN No
VPS11_HUMAN No
VPS28_HUMAN No
VPS36_HUMAN No
VRK3_HUMAN No
PMID Article Title
9019400 The TSG101 tumor susceptibility gene is located in chromosome 11 band p15 and is mutated in human breast cancer.
9242438 Aberrant splicing but not mutations of TSG101 in human breast cancer.
9366528 Aberrant splicing of the TSG101 and FHIT genes occurs frequently in multiple malignancies and in normal tissues and mimics alterations previously described in tumours.
9840940 Genomic architecture and transcriptional activation of the mouse and human tumor susceptibility gene TSG101: common types of shorter transcripts are true alternative splice variants.
10888872 DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a complex at replication foci.
11427703 Tsg101, a homologue of ubiquitin-conjugating (E2) enzymes, binds the L domain in HIV type 1 Pr55(Gag).
11595185 Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding.
11916981 Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal protein-ubiquitin conjugates.
12006492 Structure and functional interactions of the Tsg101 UEV domain.
12379843 Structure of the Tsg101 UEV domain in complex with the PTAP motif of the HIV-1 p6 protein.
12559917 Ebola virus matrix protein VP40 interaction with human cellular factors Tsg101 and Nedd4.
12802020 TSG101 interaction with HRS mediates endosomal trafficking and receptor down-regulation.
12900394 HIV Gag mimics the Tsg101-recruiting activity of the human Hrs protein.
14505570 The protein network of HIV budding.
14519844 Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins.
14581525 PPPYVEPTAP motif is the late domain of human T-cell leukemia virus type 1 Gag and mediates its functional interaction with cellular proteins Nedd4 and Tsg101.
15039775 Interactions of GGA3 with the ubiquitin sorting machinery.
15053872 Ubiquitin recognition by the human TSG101 protein.
15143060 The trihelical bundle subdomain of the GGA proteins interacts with multiple partners through overlapping but distinct sites.
15218037 The human endosomal sorting complex required for transport (ESCRT-I) and its role in HIV-1 budding.
15256501 Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding.
15489334 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
15509564 Identification of human VPS37C, a component of endosomal sorting complex required for transport-I important for viral budding.
15858022 Identification of domains in gag important for prototypic foamy virus egress.
16552148 Structure of human TSG101 UEV domain.
16571837 Cellular factors required for Lassa virus budding.
17229889 Spongiform neurodegeneration-associated E3 ligase Mahogunin ubiquitylates TSG101 and regulates endosomal trafficking.
17556548 Parallels between cytokinesis and retroviral budding: a role for the ESCRT machinery.
17853893 Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis.
18005716 Identification of human MVB12 proteins as ESCRT-I subunits that function in HIV budding.
18256029 Identification of Alix-type and non-Alix-type ALG-2-binding sites in human phospholipid scramblase 3: differential binding to an alternatively spliced isoform and amino acid-substituted mutants.
19413330 Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.
19520058 Penta-EF-hand protein ALG-2 functions as a Ca2+-dependent adaptor that bridges Alix and TSG101.
19703557 Abnormal regulation of TSG101 in mice with spongiform neurodegeneration.
21070952 Crystallographic and functional analysis of the ESCRT-I /HIV-1 Gag PTAP interaction.
21191027 Multiple interactions between the ESCRT machinery and arrestin-related proteins: implications for PPXY-dependent budding.
21269460 Initial characterization of the human central proteome.
21643473 Elucidation of new binding interactions with the tumor susceptibility gene 101 (Tsg101) protein using modified HIV-1 Gag-p6 derived peptide ligands.
21757351 UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting.
22315426 Formation and release of arrestin domain-containing protein 1-mediated microvesicles (ARMMs) at plasma membrane by recruitment of TSG101 protein.
22660413 Syndecan-syntenin-ALIX regulates the biogenesis of exosomes.
22814378 N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.
23166352 Charcot-Marie-Tooth disease-linked protein SIMPLE functions with the ESCRT machinery in endosomal trafficking.
23186163 Toward a comprehensive characterization of a human cancer cell phosphoproteome.
25944712 N-terminome analysis of the human mitochondrial proteome.
No results found.
Domain Name Domain ID Source
UEV_N IPR008883 InterPro
UBQ-conjugating_enzyme/RWD IPR016135 InterPro
SB_dom IPR017916 InterPro
ESCRT_assembly_dom IPR037202 InterPro
UEV PF05743 Pfam
Vps23_core PF09454 Pfam
SB PS51312 PROSITE
UEV PS51322 PROSITE
SSF140111 SSF140111 SUPFAM
SSF54495 SSF54495 SUPFAM