Protein: Q8WUM4

UniprotKB AC UniprotKB ID Gene name Full name Species Curated set
Q8WUM4 (Uniprot) PDC6I_HUMAN PDCD6IP Programmed cell death 6-interacting protein human No
Uniprot: Multifunctional protein involved in endocytosis, multivesicular body biogenesis, membrane repair, cytokinesis, apoptosis and maintenance of tight junction integrity. Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complexes (PubMed:14739459). The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis (PubMed:17853893, PubMed:17556548). Adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis (PubMed:17853893, PubMed:17556548, PubMed:18641129). May play a role in the regulation of both apoptosis and cell proliferation. Regulates exosome biogenesis in concert with SDC1/4 and SDCBP (PubMed:22660413). By interacting with F-actin, PARD3 and TJP1 secures the proper assembly and positioning of actomyosin-tight junction complex at the apical sides of adjacent epithelial cells that defines a spatial membrane domain essential for the maintenance of epithelial cell polarity and barrier (By similarity).(Microbial infection) Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function requires the interaction with CHMP4B. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as enveloped virus budding (HIV-1 and other lentiviruses). more..
MoonDB Dissimilar Functions
GO ID 1 Function 1 Module ID 1 GO ID 2 Function 2 Module ID 2 Association Probability (PrOnto) Interaction Probability (PrOnto)
GO:0048856 anatomical structure development 186 GO:0009056 catabolic process 48 1.47e-03 3.62e-65
Biological Processes
Module ID (MoonGO) GO ID (BP) GO Name
48 GO:0044248 cellular catabolic process
48 GO:0070647 protein modification by small protein conjugation or removal
48 GO:0006508 proteolysis
48 GO:0060255 regulation of macromolecule metabolic process
48 GO:0007165 signal transduction
186 GO:0048468 cell development
186 GO:0007166 cell surface receptor signaling pathway
186 GO:0044267 cellular protein metabolic process
186 GO:0035556 intracellular signal transduction
186 GO:0030182 neuron differentiation
186 GO:0006796 phosphate-containing compound metabolic process
186 GO:0120036 plasma membrane bounded cell projection organization
186 GO:0060255 regulation of macromolecule metabolic process
186 GO:0009966 regulation of signal transduction
215 GO:0044267 cellular protein metabolic process
215 GO:0007167 enzyme linked receptor protein signaling pathway
215 GO:0010467 gene expression
215 GO:0016310 phosphorylation
215 GO:1902531 regulation of intracellular signal transduction
215 GO:0060255 regulation of macromolecule metabolic process
403 GO:0060255 regulation of macromolecule metabolic process
699 GO:0022607 cellular component assembly
699 GO:0051649 establishment of localization in cell
699 GO:0071705 nitrogen compound transport
699 GO:0008104 protein localization
699 GO:0019538 protein metabolic process
Cellular Components
Module ID (MoonGO) GO ID (CC) GO Name
48 GO:0031410 cytoplasmic vesicle
48 GO:0012505 endomembrane system
48 GO:0005634 nucleus
48 GO:0005886 plasma membrane
186 GO:0005829 cytosol
186 GO:0005886 plasma membrane
186 GO:0031982 vesicle
215 GO:0012505 endomembrane system
215 GO:0005886 plasma membrane
215 GO:0031982 vesicle
403 GO:0005634 nucleus
699 GO:0005829 cytosol
699 GO:0012505 endomembrane system
699 GO:0043232 intracellular non-membrane-bounded organelle
699 GO:0005634 nucleus
699 GO:0031982 vesicle
GO ID (BP) GO Name Evidence Code (GO EC)
GO:0000281 mitotic cytokinesis IDA
GO:0000915 actomyosin contractile ring assembly ISS
GO:0000920 cell separation after cytokinesis IMP
GO:0006915 apoptotic process IEA
GO:0006997 nucleus organization IMP
GO:0007080 mitotic metaphase plate congression IMP
GO:0010824 regulation of centrosome duplication IMP
GO:0015031 protein transport IEA
GO:0019058 viral life cycle TAS
GO:0036258 multivesicular body assembly NAS
GO:0039702 viral budding via host ESCRT complex IGI
GO:0045199 maintenance of epithelial cell apical/basal polarity ISS
GO:0046755 viral budding IDA
GO:0051260 protein homooligomerization IDA
GO:0070830 bicellular tight junction assembly ISS
GO:0090559 regulation of membrane permeability ISS
GO:0090611 ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway IMP
GO:1901673 regulation of mitotic spindle assembly IMP
GO:1903543 positive regulation of exosomal secretion IMP
GO:1903551 regulation of extracellular exosome assembly IMP
GO:1903553 positive regulation of extracellular exosome assembly IMP
GO ID (CC) GO Name Evidence Code (GO EC)
GO:0001772 immunological synapse IDA
GO:0005815 microtubule organizing center IEA
GO:0005829 cytosol TAS
GO:0005923 bicellular tight junction IEA
GO:0005925 focal adhesion IDA
GO:0016020 membrane IDA
GO:0042470 melanosome IEA
GO:0042641 actomyosin ISS
GO:0043209 myelin sheath IEA
GO:0070062 extracellular exosome IDA
GO:0070971 endoplasmic reticulum exit site IMP
GO:0090543 Flemming body IDA
GO:1903561 extracellular vesicle IDA
No pairs of PrOnto dissimilar CC GO terms found.
PMID Article Title
10718198 Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro.
11683497 Overexpression of Hp95 induces G1 phase arrest in confluent HeLa cells.
12860994 The ALG-2-interacting protein Alix associates with CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting.
14505569 AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding.
14505570 The protein network of HIV budding.
14519844 Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins.
14583093 Structure and function of human Vps20 and Snf7 proteins.
14678797 CHMP4b is a major binding partner of the ALG-2-interacting protein Alix among the three CHMP4 isoforms.
14702039 Complete sequencing and characterization of 21,243 full-length human cDNAs.
14739459 Role of LBPA and Alix in multivesicular liposome formation and endosome organization.
15489334 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
15849434 Identification of Rab GTPase-activating protein-like protein (RabGAPLP) as a novel Alix/AIP1-interacting protein.
15908698 Tsg101 and Alix interact with murine leukemia virus Gag and cooperate with Nedd4 ubiquitin ligases during budding.
16641997 The DNA sequence, annotation and analysis of human chromosome 3.
16957052 The Ca2+-binding protein ALG-2 is recruited to endoplasmic reticulum exit sites by Sec31A and stabilizes the localization of Sec31A.
17081065 Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes.
17277784 Structural basis for viral late-domain binding to Alix.
17350572 Structural and biochemical studies of ALIX/AIP1 and its role in retrovirus budding.
17428861 Potent rescue of human immunodeficiency virus type 1 late domain mutants by ALIX/AIP1 depends on its CHMP4 binding site.
17556548 Parallels between cytokinesis and retroviral budding: a role for the ESCRT machinery.
17853893 Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis.
18066081 Structural and functional studies of ALIX interactions with YPX(n)L late domains of HIV-1 and EIAV.
18256029 Identification of Alix-type and non-Alix-type ALG-2-binding sites in human phospholipid scramblase 3: differential binding to an alternatively spliced isoform and amino acid-substituted mutants.
18511562 ALIX-CHMP4 interactions in the human ESCRT pathway.
18641129 Differential requirements for Alix and ESCRT-III in cytokinesis and HIV-1 release.
18669648 A quantitative atlas of mitotic phosphorylation.
18940611 Structural basis for Ca2+ -dependent formation of ALG-2/Alix peptide complex: Ca2+/EF3-driven arginine switch mechanism.
18948538 Midbody targeting of the ESCRT machinery by a noncanonical coiled coil in CEP55.
19413330 Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.
19520058 Penta-EF-hand protein ALG-2 functions as a Ca2+-dependent adaptor that bridges Alix and TSG101.
19608861 Lysine acetylation targets protein complexes and co-regulates major cellular functions.
19906316 Discovery of novel human transcript variants by analysis of intronic single-block EST with polyadenylation site.
20068231 Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.
20494825 Mutation of tyrosine residue 857 in the PDGF beta-receptor affects cell proliferation but not migration.
21191027 Multiple interactions between the ESCRT machinery and arrestin-related proteins: implications for PPXY-dependent budding.
21269460 Initial characterization of the human central proteome.
22073189 Exome-sequencing confirms DNAJC5 mutations as cause of adult neuronal ceroid-lipofuscinosis.
22223895 Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features.
22660413 Syndecan-syntenin-ALIX regulates the biogenesis of exosomes.
23186163 Toward a comprehensive characterization of a human cancer cell phosphoproteome.
24129315 Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.
24275569 An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.
25944712 N-terminome analysis of the human mitochondrial proteome.
No results found.
Domain Name Domain ID Source
BRO1_dom IPR004328 InterPro
ALIX_V_dom IPR025304 InterPro
BRO1 PF03097 Pfam
ALIX_LYPXL_bnd PF13949 Pfam
BRO1 PS51180 PROSITE
BRO1 SM01041 SMART