Protein: P61086

UniprotKB AC UniprotKB ID Gene name Full name Species Curated set
P61086 (Uniprot) UBE2K_HUMAN UBE2K Ubiquitin-conjugating enzyme E2 K human No
Uniprot: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1. more..
MoonDB Dissimilar Functions
GO ID 1 Function 1 Module ID 1 GO ID 2 Function 2 Module ID 2 Association Probability (PrOnto) Interaction Probability (PrOnto)
GO:0009056 catabolic process 125 GO:0009059 macromolecule biosynthetic process 384 6.37e-04 1.88e-40
Biological Processes
Module ID (MoonGO) GO ID (BP) GO Name
125 GO:0044248 cellular catabolic process
125 GO:0016567 protein ubiquitination
125 GO:0060255 regulation of macromolecule metabolic process
375 GO:0070647 protein modification by small protein conjugation or removal
375 GO:0060255 regulation of macromolecule metabolic process
384 GO:0034645 cellular macromolecule biosynthetic process
384 GO:0006464 cellular protein modification process
384 GO:0090304 nucleic acid metabolic process
384 GO:0031326 regulation of cellular biosynthetic process
384 GO:0060255 regulation of macromolecule metabolic process
384 GO:0007165 signal transduction
Cellular Components
Module ID (MoonGO) GO ID (CC) GO Name
125 GO:0005829 cytosol
125 GO:0005634 nucleus
375 GO:0005829 cytosol
375 GO:0005634 nucleus
384 GO:0005829 cytosol
384 GO:0031981 nuclear lumen
642 GO:0005634 nucleus
GO ID (BP) GO Name Evidence Code (GO EC)
GO:0006511 ubiquitin-dependent protein catabolic process TAS
GO:0010800 positive regulation of peptidyl-threonine phosphorylation IDA
GO:0010994 free ubiquitin chain polymerization IDA
GO:0016567 protein ubiquitination TAS
GO:0032434 regulation of proteasomal ubiquitin-dependent protein catabolic process IEA
GO:0035458 cellular response to interferon-beta IMP
GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process IBA
GO:0060340 positive regulation of type I interferon-mediated signaling pathway IMP
GO:0070059 intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress IEA
GO:0070936 protein K48-linked ubiquitination IDA
GO ID (CC) GO Name Evidence Code (GO EC)
GO:0005634 nucleus IBA
GO:0005737 cytoplasm IBA
GO:0005829 cytosol TAS
GO:0032433 filopodium tip IEA
GO:0070062 extracellular exosome IDA
GO ID 1 Component 1 GO ID 2 Component 2 Association Probability (PrOnto) Interaction Probability (PrOnto)
GO:0005634 nucleus GO:0070062 extracellular exosome 6.30e-13 4.54e-26
PMID Article Title
8702625 Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme.
10634809 Induction of ubiquitin-conjugating enzyme by aggregated low density lipoprotein in human macrophages and its implications for atherosclerosis.
10675012 Regulation of macrophage-specific gene expression by degenerated lipoproteins.
12665801 Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.
14702039 Complete sequencing and characterization of 21,243 full-length human cDNAs.
15489334 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
15815621 Generation and annotation of the DNA sequences of human chromosomes 2 and 4.
16714285 NARF, an nemo-like kinase (NLK)-associated ring finger protein regulates the ubiquitylation and degradation of T cell factor/lymphoid enhancer factor (TCF/LEF).
16868077 E2-25K mediates US11-triggered retro-translocation of MHC class I heavy chains in a permeabilized cell system.
17873885 E2-BRCA1 RING interactions dictate synthesis of mono- or specific polyubiquitin chain linkages.
19407372 Structure of full-length ubiquitin-conjugating enzyme E2-25K (Huntingtin-interacting protein 2).
19608861 Lysine acetylation targets protein complexes and co-regulates major cellular functions.
19906396 Destabilization of Rb by human papillomavirus E7 is cell cycle dependent: E2-25K is involved in the proteolysis.
20061386 The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines.
21269460 Initial characterization of the human central proteome.
21532592 UBCH7 reactivity profile reveals parkin and HHARI to be RING/HECT hybrids.
22223895 Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features.
22814378 N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.
23186163 Toward a comprehensive characterization of a human cancer cell phosphoproteome.
25114211 Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli.
25944712 N-terminome analysis of the human mitochondrial proteome.
No results found.
Domain Name Domain ID Source
UBQ-conjugat_E2 IPR000608 InterPro
UBA-like_sf IPR009060 InterPro
UBA IPR015940 InterPro
UBQ-conjugating_enzyme/RWD IPR016135 InterPro
UBQ-conjugating_AS IPR023313 InterPro
UQ_con PF00179 Pfam
UBA PF00627 Pfam
UBIQUITIN_CONJUGAT_1 PS00183 PROSITE
UBA PS50030 PROSITE
UBIQUITIN_CONJUGAT_2 PS50127 PROSITE
UBA SM00165 SMART
SSF46934 SSF46934 SUPFAM
SSF54495 SSF54495 SUPFAM
UBCc cd00195 CDD