Protein: Q13153

UniprotKB AC UniprotKB ID Gene name Full name Species Curated set
Q13153 (Uniprot) PAK1_HUMAN PAK1 Serine/threonine-protein kinase PAK 1 human No
Uniprot: Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Can directly phosphorylate BAD and protects cells against apoptosis. Activated by interaction with CDC42 and RAC1. Functions as GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway. Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases. Involved in the reorganization of the actin cytoskeleton, actin stress fibers and of focal adhesion complexes. Phosphorylates the tubulin chaperone TBCB and thereby plays a role in the regulation of microtubule biogenesis and organization of the tubulin cytoskeleton. Plays a role in the regulation of insulin secretion in response to elevated glucose levels. Part of a ternary complex that contains PAK1, DVL1 and MUSK that is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Phosphorylates MYL9/MLC2. Phosphorylates RAF1 at 'Ser-338' and 'Ser-339' resulting in: activation of RAF1, stimulation of RAF1 translocation to mitochondria, phosphorylation of BAD by RAF1, and RAF1 binding to BCL2. Phosphorylates SNAI1 at 'Ser-246' promoting its transcriptional repressor activity by increasing its accumulation in the nucleus. In podocytes, promotes NR3C2 nuclear localization. Required for atypical chemokine receptor ACKR2-induced phosphorylation of LIMK1 and cofilin (CFL1) and for the up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3, maybe through CFL1 phosphorylation and inactivation. Plays a role in RUFY3-mediated facilitating gastric cancer cells migration and invasion (PubMed:25766321). more..
MoonDB Dissimilar Functions
GO ID 1 Function 1 Module ID 1 GO ID 2 Function 2 Module ID 2 Association Probability (PrOnto) Interaction Probability (PrOnto)
GO:0010646 regulation of cell communication 284 GO:0006139 nucleobase-containing compound metabolic process 472 6.71e-28 7.52e-07
GO:0006139 nucleobase-containing compound metabolic process 472 GO:0033036 macromolecule localization 517 4.58e-19 9.70e-72
Biological Processes
Module ID (MoonGO) GO ID (BP) GO Name
2 GO:0044267 cellular protein metabolic process
2 GO:0010467 gene expression
2 GO:0006139 nucleobase-containing compound metabolic process
2 GO:0060255 regulation of macromolecule metabolic process
2 GO:0007165 signal transduction
218 GO:0060255 regulation of macromolecule metabolic process
284 GO:0009966 regulation of signal transduction
472 GO:0034654 nucleobase-containing compound biosynthetic process
472 GO:0060255 regulation of macromolecule metabolic process
517 GO:0008104 protein localization
517 GO:0019538 protein metabolic process
Cellular Components
Module ID (MoonGO) GO ID (CC) GO Name
2 GO:0005829 cytosol
2 GO:0005634 nucleus
218 GO:0005634 nucleus
284 GO:0005829 cytosol
284 GO:0005886 plasma membrane
472 GO:0043232 intracellular non-membrane-bounded organelle
472 GO:0005634 nucleus
517 GO:0005829 cytosol
517 GO:0012505 endomembrane system
517 GO:0031982 vesicle
GO ID (BP) GO Name Evidence Code (GO EC)
GO:0000165 MAPK cascade IDA
GO:0001666 response to hypoxia IEA
GO:0001934 positive regulation of protein phosphorylation IMP
GO:0002223 stimulatory C-type lectin receptor signaling pathway TAS
GO:0006468 protein phosphorylation IDA
GO:0006887 exocytosis IEA
GO:0006915 apoptotic process IEA
GO:0007266 Rho protein signal transduction IBA
GO:0007346 regulation of mitotic cell cycle IBA
GO:0008284 positive regulation of cell proliferation IMP
GO:0010763 positive regulation of fibroblast migration IEA
GO:0021549 cerebellum development IEA
GO:0030010 establishment of cell polarity IEA
GO:0030335 positive regulation of cell migration IDA
GO:0031116 positive regulation of microtubule polymerization IMP
GO:0031295 T cell costimulation TAS
GO:0031532 actin cytoskeleton reorganization IDA
GO:0032147 activation of protein kinase activity IBA
GO:0032869 cellular response to insulin stimulus IEA
GO:0033138 positive regulation of peptidyl-serine phosphorylation IDA
GO:0033148 positive regulation of intracellular estrogen receptor signaling pathway IDA
GO:0038095 Fc-epsilon receptor signaling pathway TAS
GO:0038096 Fc-gamma receptor signaling pathway involved in phagocytosis TAS
GO:0042060 wound healing IMP
GO:0042981 regulation of apoptotic process IBA
GO:0043507 positive regulation of JUN kinase activity IMP
GO:0045773 positive regulation of axon extension IEA
GO:0046777 protein autophosphorylation IDA
GO:0048012 hepatocyte growth factor receptor signaling pathway IMP
GO:0048013 ephrin receptor signaling pathway TAS
GO:0048754 branching morphogenesis of an epithelial tube IMP
GO:0048812 neuron projection morphogenesis ISS
GO:0050770 regulation of axonogenesis IBA
GO:0050852 T cell receptor signaling pathway TAS
GO:0051496 positive regulation of stress fiber assembly IMP
GO:0060244 negative regulation of cell proliferation involved in contact inhibition IMP
GO:0061052 negative regulation of cell growth involved in cardiac muscle cell development IEA
GO:0090314 positive regulation of protein targeting to membrane IEA
GO:1904707 positive regulation of vascular smooth muscle cell proliferation IEA
GO:1904754 positive regulation of vascular associated smooth muscle cell migration IEA
GO:2001275 positive regulation of glucose import in response to insulin stimulus IEA
GO ID (CC) GO Name Evidence Code (GO EC)
GO:0001726 ruffle IDA
GO:0005737 cytoplasm IDA
GO:0005829 cytosol TAS
GO:0005886 plasma membrane TAS
GO:0005911 cell-cell junction IDA
GO:0005925 focal adhesion IEA
GO:0014704 intercalated disc ISS
GO:0030018 Z disc ISS
GO:0030027 lamellipodium IEA
GO:0030424 axon ISS
GO:0030425 dendrite ISS
GO:0031941 filamentous actin NAS
GO:0031965 nuclear membrane ISS
GO:0032587 ruffle membrane IEA
GO:0043234 protein complex IDA
GO:0071437 invadopodium IDA
No pairs of PrOnto dissimilar CC GO terms found.
PMID Article Title
8805275 Human Ste20 homologue hPAK1 links GTPases to the JNK MAP kinase pathway.
9032240 Expression of constitutively active alpha-PAK reveals effects of the kinase on actin and focal complexes.
9395435 Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells.
9528787 A conserved negative regulatory region in alphaPAK: inhibition of PAK kinases reveals their morphological roles downstream of Cdc42 and Rac1.
9774440 Differential effects of PAK1-activating mutations reveal activity-dependent and -independent effects on cytoskeletal regulation.
10026169 Identification of Grb4/Nckbeta, a src homology 2 and 3 domain-containing adapter protein having similar binding and biological properties to Nck.
10551809 Identification of a central phosphorylation site in p21-activated kinase regulating autoinhibition and kinase activity.
10975528 Structure of PAK1 in an autoinhibited conformation reveals a multistage activation switch.
10995762 p21-activated kinase (PAK1) is phosphorylated and activated by 3-phosphoinositide-dependent kinase-1 (PDK1).
11733498 Interaction between active Pak1 and Raf-1 is necessary for phosphorylation and activation of Raf-1.
11804587 Pak1 kinase homodimers are autoinhibited in trans and dissociated upon activation by Cdc42 and Rac1.
12624090 The C-terminal kinase domain of the p34cdc2-related PITSLRE protein kinase (p110C) associates with p21-activated kinase 1 and inhibits its activity during anoikis.
12876277 PAK1 phosphorylation of MEK1 regulates fibronectin-stimulated MAPK activation.
14585966 Akt phosphorylation of serine 21 on Pak1 modulates Nck binding and cell migration.
15169762 The Down syndrome cell adhesion molecule (DSCAM) interacts with and activates Pak.
15489334 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
15611088 Integrin engagement differentially modulates epithelial cell motility by RhoA/ROCK and PAK1.
15831477 p21-activated kinase 1 regulates microtubule dynamics by phosphorylating tubulin cofactor B.
15833848 Pak1 phosphorylation of snail, a master regulator of epithelial-to-mesenchyme transition, modulates snail's subcellular localization and functions.
15893667 The active conformation of the PAK1 kinase domain.
16061695 Essential role of CIB1 in regulating PAK1 activation and cell migration.
16101281 Structural analysis of the SH3 domain of beta-PIX and its interaction with alpha-p21 activated kinase (PAK).
16278681 CRIPak, a novel endogenous Pak1 inhibitor.
16554811 Human chromosome 11 DNA sequence and analysis including novel gene identification.
16964243 A probability-based approach for high-throughput protein phosphorylation analysis and site localization.
17726028 JAK2 tyrosine kinase phosphorylates PAK1 and regulates PAK1 activity and functions.
17989089 Identification of phosphorylation sites in betaPIX and PAK1.
18325335 Affixin activates Rac1 via betaPIX in C2C12 myoblast.
18669648 A quantitative atlas of mitotic phosphorylation.
18716323 Scrib regulates PAK activity during the cell migration process.
19413330 Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.
19690332 Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.
21269460 Initial characterization of the human central proteome.
21406692 System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.
22153498 Structural insights into the autoactivation mechanism of p21-activated protein kinase.
22669945 Synapses of amphids defective (SAD-A) kinase promotes glucose-stimulated insulin secretion through activation of p21-activated kinase (PAK1) in pancreatic beta-Cells.
22814378 N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.
23186163 Toward a comprehensive characterization of a human cancer cell phosphoproteome.
23503467 A novel splice variant of calcium and integrin-binding protein 1 mediates protein kinase D2-stimulated tumour growth by regulating angiogenesis.
23633677 Beta-arrestin-dependent activation of the cofilin pathway is required for the scavenging activity of the atypical chemokine receptor D6.
24275569 An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.
25766321 PAK1 regulates RUFY3-mediated gastric cancer cell migration and invasion.
26940976 Glucose-regulated protein 78 (GRP78) binds directly to PIP3 phosphatase SKIP and determines its localization.
No results found.
Domain Name Domain ID Source
G01773 G01773 PIR
CRIB_dom IPR000095 InterPro
Prot_kinase_dom IPR000719 InterPro
Ser/Thr_kinase_AS IPR008271 InterPro
Kinase-like_dom_sf IPR011009 InterPro
Protein_kinase_ATP_BS IPR017441 InterPro
PAK_BD IPR033923 InterPro
CRIB_dom_sf IPR036936 InterPro
Pkinase PF00069 Pfam
PBD PF00786 Pfam
PROTEIN_KINASE_ATP PS00107 PROSITE
PROTEIN_KINASE_ST PS00108 PROSITE
PROTEIN_KINASE_DOM PS50011 PROSITE
CRIB PS50108 PROSITE
S_TKc SM00220 SMART
PBD SM00285 SMART
SSF56112 SSF56112 SUPFAM
CRIB_PAK_like cd01093 CDD