Protein: P32325

UniprotKB AC UniprotKB ID Gene name Full name Species Curated set
P32325 (Uniprot) DBF4_YEAST DBF4 DDK kinase regulatory subunit DBF4 yeast No
Uniprot: Regulatory subunit of the CDC7-DBF4 kinase, also called DBF4-dependent kinase (DDK), which is involved in cell cycle regulation of premitotic and premeiotic chromosome replication and in chromosome segregation. DDK plays an essential role in initiating DNA replication at replication origins by phosphorylating the MCM2 and MCM4 subunits of the MCM2-7 helicase complex. DBF4 recruits the catalytic subunit CDC7 to MCM2 and to origins of replication. DDK has also postreplicative functions in meiosis. DDK phosphorylates the meiosis-specific double-strand break protein MER2 for initiation of meiotic recombination. Interacts with CDC5 during meiosis to promote double-strand breaks and monopolar spindle orientation. Inhibits CDC5 activity during mitosis through direct binding to its PBD. more..
MoonDB Dissimilar Functions
GO ID 1 Function 1 Module ID 1 GO ID 2 Function 2 Module ID 2 Association Probability (PrOnto) Interaction Probability (PrOnto)
GO:0009056 catabolic process 1141 GO:0009059 macromolecule biosynthetic process 1198 3.10e-17 2.99e-04
Biological Processes
Module ID (MoonGO) GO ID (BP) GO Name
906 GO:0022402 cell cycle process
1141 GO:0044248 cellular catabolic process
1141 GO:1903047 mitotic cell cycle process
1141 GO:0090304 nucleic acid metabolic process
1141 GO:2000112 regulation of cellular macromolecule biosynthetic process
1141 GO:0010468 regulation of gene expression
1141 GO:0019219 regulation of nucleobase-containing compound metabolic process
1198 GO:0034645 cellular macromolecule biosynthetic process
1198 GO:0006464 cellular protein modification process
1198 GO:0090304 nucleic acid metabolic process
1198 GO:0060255 regulation of macromolecule metabolic process
1198 GO:0019219 regulation of nucleobase-containing compound metabolic process
Cellular Components
Module ID (MoonGO) GO ID (CC) GO Name
906 GO:0005634 nucleus
1141 GO:0044427 chromosomal part
1141 GO:0005634 nucleus
1198 GO:0043232 intracellular non-membrane-bounded organelle
1198 GO:0005634 nucleus
GO ID (BP) GO Name Evidence Code (GO EC)
GO:0001100 negative regulation of exit from mitosis IMP
GO:0006270 DNA replication initiation IPI
GO:0006279 premeiotic DNA replication IMP
GO:0007059 chromosome segregation IEA
GO:0033314 mitotic DNA replication checkpoint IGI
GO:0045860 positive regulation of protein kinase activity IDA
GO:0051301 cell division IEA
GO:0060903 positive regulation of meiosis I IMP
GO:0071902 positive regulation of protein serine/threonine kinase activity IMP
GO:1903468 positive regulation of DNA replication initiation IGI
GO ID (CC) GO Name Evidence Code (GO EC)
GO:0000775 chromosome, centromeric region IDA
GO:0000785 chromatin IDA
GO:0005634 nucleus IEA
GO:0031431 Dbf4-dependent protein kinase complex IPI
No pairs of PrOnto dissimilar CC GO terms found.
PMID Article Title
1514326 Genetic and molecular analysis of DNA43 and DNA52: two new cell-cycle genes in Saccharomyces cerevisiae.
1592236 Temperature-sensitive cdc7 mutations of Saccharomyces cerevisiae are suppressed by the DBF4 gene, which is required for the G1/S cell cycle transition.
8066465 Interaction of Dbf4, the Cdc7 protein kinase regulatory subunit, with yeast replication origins in vivo.
8474449 Cell cycle regulation of the yeast Cdc7 protein kinase by association with the Dbf4 protein.
8789263 Nucleotide sequence analysis of a 32,500 bp region of the right arm of Saccharomyces cerevisiae chromosome IV.
8943332 A novel role for Cdc5p in DNA replication.
9169867 The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
10330168 Cell cycle regulation of DNA replication initiator factor Dbf4p.
10373538 Cell cycle control of Cdc7p kinase activity through regulation of Dbf4p stability.
12441400 An N-terminal domain of Dbf4p mediates interaction with both origin recognition complex (ORC) and Rad53p and can deregulate late origin firing.
16319063 Dual role of the Cdc7-regulatory protein Dbf4 during yeast meiosis.
17018296 Cdc7-Dbf4 phosphorylates MCM proteins via a docking site-mediated mechanism to promote S phase progression.
17287358 Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry.
18245450 Cdc28-Clb5 (CDK-S) and Cdc7-Dbf4 (DDK) collaborate to initiate meiotic recombination in yeast.
18407956 A multidimensional chromatography technology for in-depth phosphoproteome analysis.
19013276 Dbf4-dependent CDC7 kinase links DNA replication to the segregation of homologous chromosomes in meiosis I.
19478884 Cdc7p-Dbf4p regulates mitotic exit by inhibiting Polo kinase.
19692334 Dbf4-Cdc7 phosphorylation of Mcm2 is required for cell growth.
20054399 The Dbf4-Cdc7 kinase promotes S phase by alleviating an inhibitory activity in Mcm4.
20170732 Methods to study kinase regulation of the replication fork helicase.
20436286 The Dbf4 motif C zinc finger promotes DNA replication and mediates resistance to genotoxic stress.
21036905 Dbf4 regulates the Cdc5 polo-like kinase through a distinct non-canonical binding interaction.
24374639 The reference genome sequence of Saccharomyces cerevisiae: Then and now.
No results found.
Domain Name Domain ID Source
Znf_DBF IPR006572 InterPro
Regulatory_Dfp1/Him1 IPR013939 InterPro
zf-DBF PF07535 Pfam
Dfp1_Him1_M PF08630 Pfam
ZF_DBF4 PS51265 PROSITE
S25371 S25371 PIR
ZnF_DBF SM00586 SMART