Protein: Q9UMS4

UniprotKB AC UniprotKB ID Gene name Full name Species Curated set
Q9UMS4 (Uniprot) PRP19_HUMAN PRPF19 Pre-mRNA-processing factor 19 human No
Uniprot: Ubiquitin-protein ligase which is a core component of several complexes mainly involved pre-mRNA splicing and DNA repair. Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex (PubMed:20595234). Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries (PubMed:21536736). The XAB2 complex, which contains PRPF19, is also involved in pre-mRNA splicing, transcription and transcription-coupled repair (PubMed:17981804). Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/DDR. It is recruited to the sites of DNA damage by the RPA complex where PRPF19 directly ubiquitinates RPA1 and RPA2. 'Lys-63'-linked polyubiquitination of the RPA complex allows the recruitment of the ATR-ATRIP complex and the activation of ATR, a master regulator of the DNA damage response (PubMed:24332808). May also play a role in DNA double-strand break (DSB) repair by recruiting the repair factor SETMAR to altered DNA (PubMed:18263876). As part of the PSO4 complex may also be involved in the DNA interstrand cross-links/ICLs repair process (PubMed:16223718). In addition, may also mediate 'Lys-48'-linked polyubiquitination of substrates and play a role in proteasomal degradation (PubMed:11435423). May play a role in the biogenesis of lipid droplets (By similarity). May play a role in neural differentiation possibly through its function as part of the spliceosome (By similarity). more..
MoonDB Dissimilar Functions
GO ID 1 Function 1 Module ID 1 GO ID 2 Function 2 Module ID 2 Association Probability (PrOnto) Interaction Probability (PrOnto)
GO:0019538 protein metabolic process 202 GO:0000377 RNA splicing, via transesterification reactions with bulged adenosine as nucleophile 353 1.88e-02 4.66e-37
Biological Processes
Module ID (MoonGO) GO ID (BP) GO Name
202 GO:0044267 cellular protein metabolic process
202 GO:0070887 cellular response to chemical stimulus
202 GO:0060255 regulation of macromolecule metabolic process
202 GO:0007165 signal transduction
353 GO:0000398 mRNA splicing, via spliceosome
Cellular Components
Module ID (MoonGO) GO ID (CC) GO Name
92 GO:0005634 nucleus
202 GO:0005829 cytosol
202 GO:0005886 plasma membrane
353 GO:0005654 nucleoplasm
GO ID (BP) GO Name Evidence Code (GO EC)
GO:0000209 protein polyubiquitination IDA
GO:0000244 spliceosomal tri-snRNP complex assembly IDA
GO:0000245 spliceosomal complex assembly IMP
GO:0000349 generation of catalytic spliceosome for first transesterification step IBA
GO:0000398 mRNA splicing, via spliceosome TAS
GO:0001833 inner cell mass cell proliferation IEA
GO:0006283 transcription-coupled nucleotide-excision repair TAS
GO:0006303 double-strand break repair via nonhomologous end joining IMP
GO:0008610 lipid biosynthetic process IEA
GO:0010498 proteasomal protein catabolic process IMP
GO:0034613 cellular protein localization IMP
GO:0045665 negative regulation of neuron differentiation IEA
GO:0048026 positive regulation of mRNA splicing, via spliceosome IEA
GO:0048711 positive regulation of astrocyte differentiation IEA
GO:0070534 protein K63-linked ubiquitination IMP
GO:0072422 signal transduction involved in DNA damage checkpoint IMP
GO ID (CC) GO Name Evidence Code (GO EC)
GO:0000974 Prp19 complex IDA
GO:0005634 nucleus IDA
GO:0005654 nucleoplasm TAS
GO:0005662 DNA replication factor A complex IDA
GO:0005737 cytoplasm IDA
GO:0005811 lipid droplet IEA
GO:0005819 spindle IEA
GO:0016020 membrane IDA
GO:0016607 nuclear speck IDA
GO:0035861 site of double-strand break IDA
GO:0071006 U2-type catalytic step 1 spliceosome IBA
GO:0071013 catalytic step 2 spliceosome IDA
GO ID 1 Component 1 GO ID 2 Component 2 Association Probability (PrOnto) Interaction Probability (PrOnto)
GO:0005634 nucleus GO:0016020 membrane 0.00e+00 4.39e-236
GO:0005654 nucleoplasm GO:0016020 membrane 5.76e-255 1.32e-206
GO:0016020 membrane GO:0016607 nuclear speck 9.49e-26 4.88e-97
PMID Article Title
10404385 Reassembling proteins and chaperones in human nuclear matrix protein fractions.
11082287 hNMP 200: a novel human common nuclear matrix protein combining structural and regulatory functions.
11435423 U box proteins as a new family of ubiquitin-protein ligases.
11991638 Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis.
12429849 Functional proteomic analysis of human nucleolus.
12960389 Role of human Pso4 in mammalian DNA repair and association with terminal deoxynucleotidyl transferase.
15489334 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
15660529 Interaction of U-box E3 ligase SNEV with PSMB4, the beta7 subunit of the 20 S proteasome.
16223718 The Pso4 mRNA splicing and DNA repair complex interacts with WRN for processing of DNA interstrand cross-links.
16332694 SNEV is an evolutionarily conserved splicing factor whose oligomerization is necessary for spliceosome assembly.
16388800 SNEV overexpression extends the life span of human endothelial cells.
17349974 Mouse homologue of yeast Prp19 interacts with mouse SUG1, the regulatory subunit of 26S proteasome.
17981804 Isolation of XAB2 complex involved in pre-mRNA splicing, transcription, and transcription-coupled repair.
18263876 Human Pso4 is a metnase (SETMAR)-binding partner that regulates metnase function in DNA repair.
18669648 A quantitative atlas of mitotic phosphorylation.
19188445 APE1/Ref-1 interacts with NPM1 within nucleoli and plays a role in the rRNA quality control process.
19413330 Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.
19608861 Lysine acetylation targets protein complexes and co-regulates major cellular functions.
19641227 Blom7alpha is a novel heterogeneous nuclear ribonucleoprotein K homology domain protein involved in pre-mRNA splicing that interacts with SNEVPrp19-Pso4.
20176811 Molecular architecture of the human Prp19/CDC5L complex.
20595234 The Prp19 complex and the Usp4Sart3 deubiquitinating enzyme control reversible ubiquitination at the spliceosome.
21269460 Initial characterization of the human central proteome.
21536736 The RNA polymerase II C-terminal domain promotes splicing activation through recruitment of a U2AF65-Prp19 complex.
22223895 Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features.
22961380 Human CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly.
24332808 PRP19 transforms into a sensor of RPA-ssDNA after DNA damage and drives ATR activation via a ubiquitin-mediated circuitry.
24718257 Small kinetochore associated protein (SKAP) promotes UV-induced cell apoptosis through negatively regulating pre-mRNA processing factor 19 (Prp19).
No results found.
Domain Name Domain ID Source
Ricin_B_lectin IPR000772 InterPro
WD40_repeat IPR001680 InterPro
Ubox_domain IPR003613 InterPro
Znf_RING/FYVE/PHD IPR013083 InterPro
Pre-mRNA_splic_Prp19 IPR013915 InterPro
WD40/YVTN_repeat-like_dom_sf IPR015943 InterPro
WD40_repeat_dom IPR017986 InterPro
WD40_repeat_CS IPR019775 InterPro
G-protein_beta_WD-40_rep IPR020472 InterPro
WD40_repeat_dom_sf IPR036322 InterPro
WD40 PF00400 Pfam
U-box PF04564 Pfam
Prp19 PF08606 Pfam
GPROTEINBRPT PR00320 PRINTS
WD_REPEATS_1 PS00678 PROSITE
WD_REPEATS_2 PS50082 PROSITE
WD_REPEATS_REGION PS50294 PROSITE
U_BOX PS51698 PROSITE
WD40 SM00320 SMART
Ubox SM00504 SMART
SSF50978 SSF50978 SUPFAM