Protein: Q00613

UniprotKB AC UniprotKB ID Gene name Full name Species Curated set
Q00613 (Uniprot) HSF1_HUMAN HSF1 Heat shock factor protein 1 human No
Uniprot: Function as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones heat shock proteins (HSPs) that protect cells from cellular insults' damage (PubMed:1871105, PubMed:11447121, PubMed:1986252, PubMed:7760831, PubMed:7623826, PubMed:8946918, PubMed:8940068, PubMed:9341107, PubMed:9121459, PubMed:9727490, PubMed:9499401, PubMed:9535852, PubMed:12659875, PubMed:12917326, PubMed:15016915, PubMed:25963659, PubMed:26754925). In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form (PubMed:9727490, PubMed:11583998, PubMed:16278218). Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site-specific heat shock elements (HSEs) present in the promoter regions of HSP genes (PubMed:1871105, PubMed:1986252, PubMed:8455624, PubMed:7935471, PubMed:7623826, PubMed:8940068, PubMed:9727490, PubMed:9499401, PubMed:10359787, PubMed:11583998, PubMed:12659875, PubMed:16278218, PubMed:25963659, PubMed:26754925). Activation is reversible, and during the attenuation and recovery phase period of the HSR, returns to its unactivated form (PubMed:11583998, PubMed:16278218). Binds to inverted 5'-NGAAN-3' pentamer DNA sequences (PubMed:1986252, PubMed:26727489). Binds to chromatin at heat shock gene promoters (PubMed:25963659). Plays also several other functions independently of its transcriptional activity. Involved in the repression of Ras-induced transcriptional activation of the c-fos gene in heat-stressed cells (PubMed:9341107). Positively regulates pre-mRNA 3'-end processing and polyadenylation of HSP70 mRNA upon heat-stressed cells in a symplekin (SYMPK)-dependent manner (PubMed:14707147). Plays a role in nuclear export of stress-induced HSP70 mRNA (PubMed:17897941). Plays a role in the regulation of mitotic progression (PubMed:18794143). Plays also a role as a negative regulator of non-homologous end joining (NHEJ) repair activity in a DNA damage-dependent manner (PubMed:26359349). Involved in stress-induced cancer cell proliferation in a IER5-dependent manner (PubMed:26754925).(Microbial infection) Plays a role in latent human immunodeficiency virus (HIV-1) transcriptional reactivation. Binds to the HIV-1 long terminal repeat promoter (LTR) to reactivate viral transcription by recruiting cellular transcriptional elongation factors, such as CDK9, CCNT1 and EP300. more..
MoonDB Dissimilar Functions
GO ID 1 Function 1 Module ID 1 GO ID 2 Function 2 Module ID 2 Association Probability (PrOnto) Interaction Probability (PrOnto)
GO:0009056 catabolic process 125 GO:0009059 macromolecule biosynthetic process 192, 211, 833 6.37e-04 1.88e-40
Biological Processes
Module ID (MoonGO) GO ID (BP) GO Name
125 GO:0044248 cellular catabolic process
125 GO:0016567 protein ubiquitination
125 GO:0060255 regulation of macromolecule metabolic process
177 GO:0044267 cellular protein metabolic process
192 GO:0034645 cellular macromolecule biosynthetic process
192 GO:0006464 cellular protein modification process
192 GO:0010467 gene expression
192 GO:0090304 nucleic acid metabolic process
192 GO:0009893 positive regulation of metabolic process
192 GO:0060255 regulation of macromolecule metabolic process
192 GO:0007165 signal transduction
211 GO:0034645 cellular macromolecule biosynthetic process
211 GO:0044271 cellular nitrogen compound biosynthetic process
211 GO:0090304 nucleic acid metabolic process
211 GO:0060255 regulation of macromolecule metabolic process
609 GO:0007166 cell surface receptor signaling pathway
609 GO:0006464 cellular protein modification process
609 GO:0071310 cellular response to organic substance
609 GO:0031325 positive regulation of cellular metabolic process
609 GO:0010604 positive regulation of macromolecule metabolic process
609 GO:0051173 positive regulation of nitrogen compound metabolic process
609 GO:0051246 regulation of protein metabolic process
609 GO:0006355 regulation of transcription, DNA-templated
833 GO:0034645 cellular macromolecule biosynthetic process
833 GO:0006464 cellular protein modification process
833 GO:0006796 phosphate-containing compound metabolic process
833 GO:0060255 regulation of macromolecule metabolic process
Cellular Components
Module ID (MoonGO) GO ID (CC) GO Name
125 GO:0005829 cytosol
125 GO:0005634 nucleus
162 GO:0005634 nucleus
177 GO:0005634 nucleus
192 GO:0005829 cytosol
192 GO:0005654 nucleoplasm
211 GO:0005829 cytosol
211 GO:0031981 nuclear lumen
609 GO:0005829 cytosol
609 GO:0043232 intracellular non-membrane-bounded organelle
609 GO:0070013 intracellular organelle lumen
609 GO:0005634 nucleus
609 GO:0005886 plasma membrane
833 GO:0005829 cytosol
GO ID (BP) GO Name Evidence Code (GO EC)
GO:0000122 negative regulation of transcription from RNA polymerase II promoter IDA
GO:0000165 MAPK cascade IDA
GO:0001892 embryonic placenta development IEA
GO:0006281 DNA repair IEA
GO:0006397 mRNA processing IEA
GO:0006952 defense response IEA
GO:0007143 female meiotic nuclear division IEA
GO:0007283 spermatogenesis IEA
GO:0007584 response to nutrient IEA
GO:0008284 positive regulation of cell proliferation IMP
GO:0008285 negative regulation of cell proliferation IEA
GO:0009299 mRNA transcription IDA
GO:0010667 negative regulation of cardiac muscle cell apoptotic process IEA
GO:0014823 response to activity IEA
GO:0032720 negative regulation of tumor necrosis factor production IEA
GO:0033574 response to testosterone IEA
GO:0034605 cellular response to heat IMP
GO:0034620 cellular response to unfolded protein IDA
GO:0035865 cellular response to potassium ion IEA
GO:0040018 positive regulation of multicellular organism growth IEA
GO:0042531 positive regulation of tyrosine phosphorylation of STAT protein IEA
GO:0043280 positive regulation of cysteine-type endopeptidase activity involved in apoptotic process IEA
GO:0043497 regulation of protein heterodimerization activity IMP
GO:0043623 cellular protein complex assembly IDA
GO:0045931 positive regulation of mitotic cell cycle IMP
GO:0045944 positive regulation of transcription from RNA polymerase II promoter IDA
GO:0051028 mRNA transport IEA
GO:0051260 protein homooligomerization IDA
GO:0060136 embryonic process involved in female pregnancy IEA
GO:0061408 positive regulation of transcription from RNA polymerase II promoter in response to heat stress IDA
GO:0070207 protein homotrimerization IDA
GO:0070301 cellular response to hydrogen peroxide IEA
GO:0071222 cellular response to lipopolysaccharide IEA
GO:0071276 cellular response to cadmium ion IDA
GO:0071280 cellular response to copper ion IDA
GO:0071392 cellular response to estradiol stimulus IEA
GO:0071480 cellular response to gamma radiation IDA
GO:0072738 cellular response to diamide IDA
GO:0090084 negative regulation of inclusion body assembly IEA
GO:0090261 positive regulation of inclusion body assembly IEA
GO:1900034 regulation of cellular response to heat TAS
GO:1900365 positive regulation of mRNA polyadenylation IMP
GO:1901215 negative regulation of neuron death IEA
GO:1902512 positive regulation of apoptotic DNA fragmentation IEA
GO:1903936 cellular response to sodium arsenite IDA
GO:1904385 cellular response to angiotensin IEA
GO:1904528 positive regulation of microtubule binding IEA
GO:1904843 cellular response to nitroglycerin IEA
GO:1904845 cellular response to L-glutamine IEA
GO:1990910 response to hypobaric hypoxia IEA
GO:1990911 response to psychosocial stress IEA
GO:2001033 negative regulation of double-strand break repair via nonhomologous end joining IMP
GO ID (CC) GO Name Evidence Code (GO EC)
GO:0000776 kinetochore IDA
GO:0000777 condensed chromosome kinetochore IEA
GO:0000791 euchromatin IEA
GO:0000792 heterochromatin IEA
GO:0005634 nucleus IDA
GO:0005654 nucleoplasm TAS
GO:0005737 cytoplasm IDA
GO:0005813 centrosome IDA
GO:0005829 cytosol TAS
GO:0016605 PML body IDA
GO:0045120 pronucleus IEA
GO:0048471 perinuclear region of cytoplasm IDA
GO:0097165 nuclear stress granule IDA
GO:0097431 mitotic spindle pole IDA
GO:1990904 ribonucleoprotein complex IDA
No pairs of PrOnto dissimilar CC GO terms found.
PMID Article Title
1871105 Molecular cloning and expression of a human heat shock factor, HSF1.
1871106 Isolation of a cDNA for HSF2: evidence for two heat shock factor genes in humans.
1986252 Heat shock-induced interactions of heat shock transcription factor and the human hsp70 promoter examined by in vivo footprinting.
7623826 Multiple layers of regulation of human heat shock transcription factor 1.
7760831 A heat shock-responsive domain of human HSF1 that regulates transcription activation domain function.
7935376 Interaction between heat shock factor and hsp70 is insufficient to suppress induction of DNA-binding activity in vivo.
7935471 Activation of the DNA-binding ability of human heat shock transcription factor 1 may involve the transition from an intramolecular to an intermolecular triple-stranded coiled-coil structure.
8455624 Activation of human heat shock genes is accompanied by oligomerization, modification, and rapid translocation of heat shock transcription factor HSF1.
8940068 Sequential phosphorylation by mitogen-activated protein kinase and glycogen synthase kinase 3 represses transcriptional activation by heat shock factor-1.
8946918 Repression of human heat shock factor 1 activity at control temperature by phosphorylation.
9121459 Repression of the heat shock factor 1 transcriptional activation domain is modulated by constitutive phosphorylation.
9222587 Evidence for a role of Hsp70 in the regulation of the heat shock response in mammalian cells.
9341107 Heat shock factor 1 represses Ras-induced transcriptional activation of the c-fos gene.
9499401 Molecular chaperones as HSF1-specific transcriptional repressors.
9535852 Transcriptional activation of heat shock factor HSF1 probed by phosphopeptide analysis of factor 32P-labeled in vivo.
9727490 Repression of heat shock transcription factor HSF1 activation by HSP90 (HSP90 complex) that forms a stress-sensitive complex with HSF1.
10359787 Rapid and reversible relocalization of heat shock factor 1 within seconds to nuclear stress granules.
10413683 Human heat shock factor 1 is predominantly a nuclear protein before and after heat stress.
10747973 c-Jun NH2-terminal kinase targeting and phosphorylation of heat shock factor-1 suppress its transcriptional activity.
11005381 Potential targets for HSF1 within the preinitiation complex.
11447121 Phosphorylation of serine 230 promotes inducible transcriptional activity of heat shock factor 1.
11514557 Regulation of heat shock transcription factor 1 by stress-induced SUMO-1 modification.
11583998 Evidence for a mechanism of repression of heat shock factor 1 transcriptional activity by a multichaperone complex.
12646186 Insights into the regulation of heat shock transcription factor 1 SUMO-1 modification.
12659875 Transcriptional activity and DNA binding of heat shock factor-1 involve phosphorylation on threonine 142 by CK2.
12665592 Phosphorylation of serine 303 is a prerequisite for the stress-inducible SUMO modification of heat shock factor 1.
12917326 Regulation of molecular chaperone gene transcription involves the serine phosphorylation, 14-3-3 epsilon binding, and cytoplasmic sequestration of heat shock factor 1.
14702039 Complete sequencing and characterization of 21,243 full-length human cDNAs.
14707147 HSF1 modulation of Hsp70 mRNA polyadenylation via interaction with symplekin.
15016915 DAXX interacts with heat shock factor 1 during stress activation and enhances its transcriptional activity.
15489334 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
15661742 Polo-like kinase 1 phosphorylates heat shock transcription factor 1 and mediates its nuclear translocation during heat stress.
15760475 Analysis of phosphorylation of human heat shock factor 1 in cells experiencing a stress.
16278218 Phosphorylation of HSF1 by MAPK-activated protein kinase 2 on serine 121, inhibits transcriptional activity and promotes HSP90 binding.
16371476 PDSM, a motif for phosphorylation-dependent SUMO modification.
16421571 DNA sequence and analysis of human chromosome 8.
16554823 RNA-mediated response to heat shock in mammalian cells.
17081983 Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.
17467953 Nine-amino-acid transactivation domain: establishment and prediction utilities.
17897941 HSF1-TPR interaction facilitates export of stress-induced HSP70 mRNA.
18220336 Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis.
18669648 A quantitative atlas of mitotic phosphorylation.
18794143 HSF1 as a mitotic regulator: phosphorylation of HSF1 by Plk1 is essential for mitotic progression.
19229036 Stress-inducible regulation of heat shock factor 1 by the deacetylase SIRT1.
19413330 Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.
19690332 Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.
20068231 Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.
21085490 Protein kinase A binds and activates heat shock factor 1.
21406692 System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.
21597468 Transformation of eEF1Bdelta into heat-shock response transcription factor by alternative splicing.
23186163 Toward a comprehensive characterization of a human cancer cell phosphoproteome.
24275569 An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.
24581496 Interplay of acetyltransferase EP300 and the proteasome system in regulating heat shock transcription factor 1.
25816751 HSF1 transcriptional activity is modulated by IER5 and PP2A/B55.
25963659 Uncoupling stress-inducible phosphorylation of heat shock factor 1 from its activation.
26159920 BAG3 affects the nucleocytoplasmic shuttling of HSF1 upon heat stress.
26359349 Heat shock factor 1, an inhibitor of non-homologous end joining repair.
26496226 Immediate-early response 5 (IER5) interacts with protein phosphatase 2A and regulates the phosphorylation of ribosomal protein S6 kinase and heat shock factor 1.
26517842 Client proteins and small molecule inhibitors display distinct binding preferences for constitutive and stress-induced HSP90 isoforms and their conformationally restricted mutants.
26727489 Structure of human heat-shock transcription factor 1 in complex with DNA.
26754925 IER5 generates a novel hypo-phosphorylated active form of HSF1 and contributes to tumorigenesis.
27189267 Heat shock factor 1 mediates latent HIV reactivation.
27354066 Heat shock factor 1 is a substrate for p38 mitogen-activated protein kinases.
28112733 Site-specific mapping of the human SUMO proteome reveals co-modification with phosphorylation.
No results found.
Domain Name Domain ID Source
A41137 A41137 PIR
HSF_DNA-bd IPR000232 InterPro
Vert_HSTF_C IPR010542 InterPro
HSF_fam IPR027725 InterPro
WH-like_DNA-bd_sf IPR036388 InterPro
WH_DNA-bd_sf IPR036390 InterPro
HSF_DNA-bind PF00447 Pfam
Vert_HS_TF PF06546 Pfam
HSFDOMAIN PR00056 PRINTS
HSF_DOMAIN PS00434 PROSITE
PTHR10015 PTHR10015 PANTHER
HSF SM00415 SMART
SSF46785 SSF46785 SUPFAM