Protein: P42566

UniprotKB AC UniprotKB ID Gene name Full name Species Curated set
P42566 (Uniprot) EPS15_HUMAN EPS15 Epidermal growth factor receptor substrate 15 human Yes
Uniprot: Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2. more..
Dissimilar Functions Manual Curation

Function 1: Involved in cell growth regulation. EGFR phosphorylation. Acts as a clathrin adapter required for post-Golgi trafficking. Condition: cytoplasm or cell membrane localisation.

Function 2: Transcription regulation. Condition: nuclear localisation (import due to its N-terminal domain, export LMB sensitive with interaction with CRM1).

Publications (PMID): 11777906, 11425879, 19814798

Biological Processes
Module ID (MoonGO) GO ID (BP) GO Name
134 GO:0044267 cellular protein metabolic process
134 GO:0060255 regulation of macromolecule metabolic process
134 GO:0007165 signal transduction
215 GO:0044267 cellular protein metabolic process
215 GO:0007167 enzyme linked receptor protein signaling pathway
215 GO:0010467 gene expression
215 GO:0016310 phosphorylation
215 GO:1902531 regulation of intracellular signal transduction
215 GO:0060255 regulation of macromolecule metabolic process
451 GO:0007165 signal transduction
451 GO:0016192 vesicle-mediated transport
Cellular Components
Module ID (MoonGO) GO ID (CC) GO Name
134 GO:0005829 cytosol
134 GO:0005634 nucleus
215 GO:0012505 endomembrane system
215 GO:0005886 plasma membrane
215 GO:0031982 vesicle
368 GO:0005829 cytosol
368 GO:0005634 nucleus
368 GO:0031982 vesicle
451 GO:0005829 cytosol
451 GO:0012505 endomembrane system
451 GO:0005634 nucleus
GO ID (BP) GO Name Evidence Code (GO EC)
GO:0001921 positive regulation of receptor recycling IEA
GO:0006895 Golgi to endosome transport IMP
GO:0007173 epidermal growth factor receptor signaling pathway TAS
GO:0008283 cell proliferation TAS
GO:0015031 protein transport IEA
GO:0016050 vesicle organization TAS
GO:0019065 receptor-mediated endocytosis of virus by host cell IMP
GO:0032456 endocytic recycling IC
GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway TAS
GO:0042127 regulation of cell proliferation IEA
GO:0046718 viral entry into host cell IMP
GO:0048268 clathrin coat assembly IDA
GO:0061024 membrane organization TAS
GO ID (CC) GO Name Evidence Code (GO EC)
GO:0005829 cytosol TAS
GO:0005886 plasma membrane IDA
GO:0005905 clathrin-coated pit TAS
GO:0009925 basal plasma membrane IEA
GO:0016020 membrane IDA
GO:0016235 aggresome IDA
GO:0016324 apical plasma membrane IEA
GO:0030122 AP-2 adaptor complex IEA
GO:0031901 early endosome membrane IEA
GO:0043231 intracellular membrane-bounded organelle IDA
GO:0060170 ciliary membrane IEA
GO ID 1 Component 1 GO ID 2 Component 2 Association Probability (PrOnto) Interaction Probability (PrOnto)
GO:0005886 plasma membrane GO:0043231 intracellular membrane-bounded organelle 0.00e+00 3.79e-47
PMID Article Title
8134107 A novel gene, AF-1p, fused to HRX in t(1;11)(p32;q23), is not related to AF-4, AF-9 nor ENL.
8183552 The human eps15 gene, encoding a tyrosine kinase substrate, is conserved in evolution and maps to 1p31-p32.
8662907 Interaction between the amino-terminal SH3 domain of CRK and its natural target proteins.
9721102 Structure and Asn-Pro-Phe binding pocket of the Eps15 homology domain.
9723620 Epsin is an EH-domain-binding protein implicated in clathrin-mediated endocytosis.
10757979 Solution structure of Eps15's third EH domain reveals coincident Phe-Trp and Asn-Pro-Phe binding sites.
11062555 Molecular mechanism of NPF recognition by EH domains.
12551915 STAM and Hrs are subunits of a multivalent ubiquitin-binding complex on early endosomes.
14702039 Complete sequencing and characterization of 21,243 full-length human cDNAs.
16159959 Ubiquilin recruits Eps15 into ubiquitin-rich cytoplasmic aggregates via a UIM-UBL interaction.
16314522 Endosomal transport of ErbB-2: mechanism for nuclear entry of the cell surface receptor.
16325581 TTP specifically regulates the internalization of the transferrin receptor.
16710414 The DNA sequence and biological annotation of human chromosome 1.
16903783 Role of the AP2 beta-appendage hub in recruiting partners for clathrin-coated vesicle assembly.
16964243 A probability-based approach for high-throughput protein phosphorylation analysis and site localization.
17525332 ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.
17974005 The full-ORF clone resource of the German cDNA consortium.
18088087 Phosphoproteome of resting human platelets.
18199683 The ubiquitin-like protein PLIC-2 is a negative regulator of G protein-coupled receptor endocytosis.
18200045 Structure of the Eps15-stonin2 complex provides a molecular explanation for EH-domain ligand specificity.
18362181 An endosomally localized isoform of Eps15 interacts with Hrs to mediate degradation of epidermal growth factor receptor.
18669648 A quantitative atlas of mitotic phosphorylation.
18691976 Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.
19413330 Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.
19458185 Endocytic accessory proteins are functionally distinguished by their differential effects on the maturation of clathrin-coated pits.
19690332 Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.
20068231 Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.
20448150 FCHo proteins are nucleators of clathrin-mediated endocytosis.
21269460 Initial characterization of the human central proteome.
21406692 System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.
21762413 Characterization of the EFC/F-BAR domain protein, FCHO2.
22223895 Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features.
22484487 Distinct and separable activities of the endocytic clathrin-coat components Fcho1/2 and AP-2 in developmental patterning.
22648170 The clathrin adaptor Dab2 recruits EH domain scaffold proteins to regulate integrin beta1 endocytosis.
22814378 N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.
23186163 Toward a comprehensive characterization of a human cancer cell phosphoproteome.
24275569 An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.
24768539 K33-linked polyubiquitination of coronin 7 by Cul3-KLHL20 ubiquitin E3 ligase regulates protein trafficking.
26822536 Structural basis for the recognition of two consecutive mutually interacting DPF motifs by the SGIP1 mu homology domain.
27670116 NPF motifs in the vaccinia virus protein A36 recruit intersectin-1 to promote Cdc42:N-WASP-mediated viral release from infected cells.
No results found.
Domain Name Domain ID Source
EH_dom IPR000261 InterPro
EF_hand_dom IPR002048 InterPro
UIM_dom IPR003903 InterPro
EF-hand-dom_pair IPR011992 InterPro
EF_Hand_1_Ca_BS IPR018247 InterPro
EF-hand_4 PF12763 Pfam
EF_HAND_1 PS00018 PROSITE
EH PS50031 PROSITE
EF_HAND_2 PS50222 PROSITE
UIM PS50330 PROSITE
S43074 S43074 PIR
EH SM00027 SMART
EFh SM00054 SMART
UIM SM00726 SMART
SSF47473 SSF47473 SUPFAM