Protein: P04406

UniprotKB AC UniprotKB ID Gene name Full name Species Curated set
P04406 (Uniprot) G3P_HUMAN GAPDH Glyceraldehyde-3-phosphate dehydrogenase human Yes
Uniprot: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. more..
Dissimilar Functions Manual Curation

Function 1: Has glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities. Condition: monomeric form.

Function 2: Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Condition: tetrameric form.

Publications (PMID): 1924305, 15877277

Biological Processes
Module ID (MoonGO) GO ID (BP) GO Name
51 GO:0060255 regulation of macromolecule metabolic process
116 GO:0019538 protein metabolic process
116 GO:0060255 regulation of macromolecule metabolic process
214 GO:0044267 cellular protein metabolic process
214 GO:0006355 regulation of transcription, DNA-templated
237 GO:0022607 cellular component assembly
237 GO:0034645 cellular macromolecule biosynthetic process
237 GO:0044271 cellular nitrogen compound biosynthetic process
237 GO:0006464 cellular protein modification process
237 GO:0051649 establishment of localization in cell
237 GO:0010467 gene expression
237 GO:0031324 negative regulation of cellular metabolic process
237 GO:0010605 negative regulation of macromolecule metabolic process
237 GO:0051172 negative regulation of nitrogen compound metabolic process
237 GO:0090304 nucleic acid metabolic process
237 GO:0033365 protein localization to organelle
237 GO:0015031 protein transport
237 GO:0050790 regulation of catalytic activity
237 GO:0051128 regulation of cellular component organization
238 GO:0060255 regulation of macromolecule metabolic process
536 GO:0010604 positive regulation of macromolecule metabolic process
536 GO:0051173 positive regulation of nitrogen compound metabolic process
536 GO:0019538 protein metabolic process
536 GO:0007165 signal transduction
Cellular Components
Module ID (MoonGO) GO ID (CC) GO Name
51 GO:0005634 nucleus
116 GO:0005829 cytosol
116 GO:0070013 intracellular organelle lumen
116 GO:0005634 nucleus
214 GO:0044428 nuclear part
237 GO:0005829 cytosol
237 GO:0012505 endomembrane system
237 GO:0005615 extracellular space
237 GO:0043232 intracellular non-membrane-bounded organelle
237 GO:0070013 intracellular organelle lumen
237 GO:0044428 nuclear part
237 GO:0031982 vesicle
238 GO:0005829 cytosol
238 GO:0005634 nucleus
536 GO:0005829 cytosol
536 GO:0005634 nucleus
GO ID (BP) GO Name Evidence Code (GO EC)
GO:0000226 microtubule cytoskeleton organization ISS
GO:0006094 gluconeogenesis TAS
GO:0010951 negative regulation of endopeptidase activity IDA
GO:0016241 regulation of macroautophagy TAS
GO:0017148 negative regulation of translation IDA
GO:0031640 killing of cells of other organism IDA
GO:0035606 peptidyl-cysteine S-trans-nitrosylation ISS
GO:0050715 positive regulation of cytokine secretion IDA
GO:0050821 protein stabilization ISS
GO:0050832 defense response to fungus IDA
GO:0051402 neuron apoptotic process ISS
GO:0051873 killing by host of symbiont cells IDA
GO:0052501 positive regulation by organism of apoptotic process in other organism involved in symbiotic interaction IDA
GO:0061621 canonical glycolysis TAS
GO:0061844 antimicrobial humoral immune response mediated by antimicrobial peptide IDA
GO:0071346 cellular response to interferon-gamma IDA
GO ID (CC) GO Name Evidence Code (GO EC)
GO:0005634 nucleus IDA
GO:0005737 cytoplasm IDA
GO:0005811 lipid droplet IDA
GO:0005829 cytosol TAS
GO:0005886 plasma membrane IDA
GO:0015630 microtubule cytoskeleton ISS
GO:0016020 membrane IDA
GO:0030529 intracellular ribonucleoprotein complex IDA
GO:0031012 extracellular matrix IDA
GO:0031965 nuclear membrane IDA
GO:0031982 vesicle IDA
GO:0043231 intracellular membrane-bounded organelle IDA
GO:0048471 perinuclear region of cytoplasm IEA
GO:0070062 extracellular exosome IDA
GO:0097452 GAIT complex IDA
GO ID 1 Component 1 GO ID 2 Component 2 Association Probability (PrOnto) Interaction Probability (PrOnto)
GO:0005634 nucleus GO:0005886 plasma membrane 5.35e-300 7.52e-165
GO:0005634 nucleus GO:0016020 membrane 0.00e+00 4.39e-236
GO:0005634 nucleus GO:0031012 extracellular matrix 1.58e-13 4.18e-03
GO:0005634 nucleus GO:0031982 vesicle 1.94e-54 3.27e-93
GO:0005634 nucleus GO:0070062 extracellular exosome 6.30e-13 4.54e-26
GO:0005829 cytosol GO:0031012 extracellular matrix 4.46e-06 5.18e-08
GO:0005886 plasma membrane GO:0015630 microtubule cytoskeleton 4.17e-10 2.15e-03
GO:0005886 plasma membrane GO:0030529 intracellular ribonucleoprotein complex 1.50e-65 9.46e-34
GO:0005886 plasma membrane GO:0043231 intracellular membrane-bounded organelle 0.00e+00 3.79e-47
PMID Article Title
957435 Twinning in crystals of human skeletal muscle D-glyceraldehyde-3-phosphate dehydrogenase.
1193541 The covalent structure of glyceraldehyde-phosphate dehydrogenase from human muscles. Isolation and amino acid sequences of peptides from tryptic digest.
1924305 A human nuclear uracil DNA glycosylase is the 37-kDa subunit of glyceraldehyde-3-phosphate dehydrogenase.
2987855 Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene.
3027061 Identification of the 37-kDa protein displaying a variable interaction with the erythroid cell membrane as glyceraldehyde-3-phosphate dehydrogenase.
3170585 Isolation and complete sequence of a functional human glyceraldehyde-3-phosphate dehydrogenase gene.
3664468 Enhanced expression of a glyceraldehyde-3-phosphate dehydrogenase gene in human lung cancers.
6096136 The glyceraldehyde 3 phosphate dehydrogenase gene family: structure of a human cDNA and of an X chromosome linked pseudogene; amazing complexity of the gene family in mouse.
6096821 The complete sequence of a full length cDNA for human liver glyceraldehyde-3-phosphate dehydrogenase: evidence for multiple mRNA species.
7030790 The complete amino acid sequence of human muscle glyceraldehyde 3-phosphate dehydrogenase.
7498159 The major protein expression profile and two-dimensional protein database of human heart.
10944468 cDNA cloning by amplification of circularized first strand cDNAs reveals non-IRE-regulated iron-responsive mRNAs.
11724794 Glyceraldehyde-3-phosphate dehydrogenase is phosphorylated by protein kinase Ciota /lambda and plays a role in microtubule dynamics in the early secretory pathway.
12665801 Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.
12829261 Subcellular localization of human glyceraldehyde-3-phosphate dehydrogenase is independent of its glycolytic function.
15479637 Noncanonical function of glutamyl-prolyl-tRNA synthetase: gene-specific silencing of translation.
15489334 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
15592455 Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.
15628863 Oxidative stress-responsive intracellular regulation specific for the angiostatic form of human tryptophanyl-tRNA synthetase.
16239728 Structural analysis of human liver glyceraldehyde-3-phosphate dehydrogenase.
16501887 The ubiquitin-specific protease USP25 interacts with three sarcomeric proteins.
16510976 High-resolution structure of human D-glyceraldehyde-3-phosphate dehydrogenase.
16541075 The finished DNA sequence of human chromosome 12.
16815975 HERC5 is an IFN-induced HECT-type E3 protein ligase that mediates type I IFN-induced ISGylation of protein targets.
17081983 Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.
18183946 Strategy for comprehensive identification of post-translational modifications in cellular proteins, including low abundant modifications: application to glyceraldehyde-3-phosphate dehydrogenase.
18669648 A quantitative atlas of mitotic phosphorylation.
19001379 FKBP36 is an inherent multifunctional glyceraldehyde-3-phosphate dehydrogenase inhibitor.
19413330 Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.
19608861 Lysine acetylation targets protein complexes and co-regulates major cellular functions.
19690332 Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.
20068231 Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.
20644585 Haponin (eIF1AD) interacts with glyceraldehyde 3-phosphate dehydrogenase in the CHO-K1 cell line.
21269460 Initial characterization of the human central proteome.
21406692 System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.
21908771 The first identification of lysine malonylation substrates and its regulatory enzyme.
22771119 Protection of extraribosomal RPL13a by GAPDH and dysregulation by S-nitrosylation.
22814378 N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.
22905912 Resveratrol-induced changes of the human adipocyte secretion profile.
23071094 Heterotrimeric GAIT complex drives transcript-selective translation inhibition in murine macrophages.
23186163 Toward a comprehensive characterization of a human cancer cell phosphoproteome.
24275569 An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.
25086035 Oxidation of an exposed methionine instigates the aggregation of glyceraldehyde-3-phosphate dehydrogenase.
25417112 Target-selective protein S-nitrosylation by sequence motif recognition.
25944712 N-terminome analysis of the human mitochondrial proteome.
28112733 Site-specific mapping of the human SUMO proteome reveals co-modification with phosphorylation.
No results found.
Domain Name Domain ID Source
DEHUG3 A31988 PIR
Glyceraldehyde-3-P_DH_1 IPR006424 InterPro
GlycerAld_3-P_DH_NAD(P)-bd IPR020828 InterPro
GlycerAld_3-P_DH_cat IPR020829 InterPro
GlycerAld_3-P_DH_AS IPR020830 InterPro
GlycerAld/Erythrose_P_DH IPR020831 InterPro
NAD(P)-bd_dom_sf IPR036291 InterPro
Gp_dh_N PF00044 Pfam
Gp_dh_C PF02800 Pfam
GAP_DH PIRSF000149 PIRSF
G3PDHDRGNASE PR00078 PRINTS
GAPDH PS00071 PROSITE
PTHR10836 PTHR10836 PANTHER
Gp_dh_N SM00846 SMART
SSF51735 SSF51735 SUPFAM
GAPDH-I TIGR01534 TIGRFAMs