Protein: P13010

UniprotKB AC UniprotKB ID Gene name Full name Species Curated set
P13010 (Uniprot) XRCC5_HUMAN XRCC5 X-ray repair cross-complementing protein 5 human Yes
Uniprot: Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together (PubMed:12145306, PubMed:20383123, PubMed:7957065, PubMed:8621488). The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression (PubMed:20383123). The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription (PubMed:8621488). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway. more..

Function 1: Single stranded DNA-dependent ATP-dependent helicase.

Function 2: Telomere maintainance in association with NARG1, the Ku p70/p86 dimer binds to the osteocalcin promoter and activates osteocalcin expression.

Publications (PMID): 12145306

Module ID (MoonGO) GO ID (BP) GO Name
404 GO:0044267 cellular protein metabolic process
404 GO:0007165 signal transduction
428 GO:0044267 cellular protein metabolic process
428 GO:0090304 nucleic acid metabolic process
428 GO:0060255 regulation of macromolecule metabolic process
663 GO:0015833 peptide transport
663 GO:0008104 protein localization
Module ID (MoonGO) GO ID (CC) GO Name
39 GO:0043232 intracellular non-membrane-bounded organelle
404 GO:0005829 cytosol
404 GO:0012505 endomembrane system
404 GO:0031982 vesicle
428 GO:0043232 intracellular non-membrane-bounded organelle
428 GO:0031981 nuclear lumen
663 GO:0005829 cytosol
663 GO:0031982 vesicle
GO ID (BP) GO Name Evidence Code (GO EC)
GO:0000723 telomere maintenance IEA
GO:0002218 activation of innate immune response IDA
GO:0006302 double-strand break repair IMP
GO:0006303 double-strand break repair via nonhomologous end joining TAS
GO:0006310 DNA recombination IEA
GO:0006351 transcription, DNA-templated IEA
GO:0007420 brain development IEA
GO:0008283 cell proliferation IEA
GO:0032204 regulation of telomere maintenance TAS
GO:0032212 positive regulation of telomere maintenance via telomerase IMP
GO:0032481 positive regulation of type I interferon production TAS
GO:0032508 DNA duplex unwinding IEA
GO:0042493 response to drug IEA
GO:0043085 positive regulation of catalytic activity TAS
GO:0043312 neutrophil degranulation TAS
GO:0045087 innate immune response IEA
GO:0045860 positive regulation of protein kinase activity IDA
GO:0045892 negative regulation of transcription, DNA-templated IMP
GO:0048660 regulation of smooth muscle cell proliferation IMP
GO:0050769 positive regulation of neurogenesis IEA
GO:0051973 positive regulation of telomerase activity TAS
GO:0060218 hematopoietic stem cell differentiation IEA
GO:0070198 protein localization to chromosome, telomeric region TAS
GO:0071398 cellular response to fatty acid IEA
GO:0071475 cellular hyperosmotic salinity response IEA
GO:0071480 cellular response to gamma radiation IDA
GO:0071481 cellular response to X-ray IEA
GO:0075713 establishment of integrated proviral latency TAS
GO:1904430 negative regulation of t-circle formation IMP
GO:1990830 cellular response to leukemia inhibitory factor IEA
GO ID (CC) GO Name Evidence Code (GO EC)
GO:0000783 nuclear telomere cap complex TAS
GO:0000784 nuclear chromosome, telomeric region IDA
GO:0005576 extracellular region TAS
GO:0005634 nucleus IDA
GO:0005654 nucleoplasm TAS
GO:0005730 nucleolus IEA
GO:0005829 cytosol TAS
GO:0005886 plasma membrane IDA
GO:0016020 membrane IDA
GO:0030529 intracellular ribonucleoprotein complex IDA
GO:0032993 protein-DNA complex IDA
GO:0034774 secretory granule lumen TAS
GO:0043234 protein complex IDA
GO:0043564 Ku70:Ku80 complex IDA
GO:0070419 nonhomologous end joining complex IDA
GO ID 1 Component 1 GO ID 2 Component 2 Association Probability (PrOnto) Interaction Probability (PrOnto)
GO:0000784 nuclear chromosome, telomeric region GO:0005886 plasma membrane 2.99e-06 4.16e-73
GO:0005576 extracellular region GO:0005634 nucleus 4.48e-91 1.28e-107
GO:0005576 extracellular region GO:0005654 nucleoplasm 6.62e-72 7.68e-98
GO:0005576 extracellular region GO:0005730 nucleolus 6.04e-15 4.85e-05
GO:0005634 nucleus GO:0005886 plasma membrane 5.35e-300 7.52e-165
GO:0005634 nucleus GO:0016020 membrane 0.00e+00 4.39e-236
GO:0005654 nucleoplasm GO:0005886 plasma membrane 9.14e-199 6.17e-196
GO:0005654 nucleoplasm GO:0016020 membrane 5.76e-255 1.32e-206
GO:0005730 nucleolus GO:0005886 plasma membrane 7.70e-47 3.09e-07
GO:0005730 nucleolus GO:0016020 membrane 8.89e-46 1.83e-10
GO:0005886 plasma membrane GO:0030529 intracellular ribonucleoprotein complex 1.50e-65 9.46e-34
GO:0005886 plasma membrane GO:0032993 protein-DNA complex 3.53e-12 4.64e-51
GO:0016020 membrane GO:0032993 protein-DNA complex 3.22e-21 3.33e-35
PMID Article Title
1537839 Identification of proteins binding to interferon-inducible transcriptional enhancers in hematopoietic cells.
2211668 Purification and characterization of proximal sequence element-binding protein 1, a transcription activating protein related to Ku and TREF that binds the proximal sequence element of the human U1 promoter.
2212941 The autoantigen Ku is indistinguishable from NF IV, a protein forming multimeric protein-DNA complexes.
2308937 Isolation and characterization of cDNA encoding the 80-kDa subunit protein of the human autoantigen Ku (p70/p80) recognized by autoantibodies from patients with scleroderma-polymyositis overlap syndrome.
2760028 cDNA-derived amino acid sequence of the 86-kDa subunit of the Ku antigen.
7882982 Purification of the sequence-specific transcription factor CTCBF, involved in the control of human collagen IV genes: subunits with homology to Ku antigen.
7957065 Human DNA helicase II: a novel DNA unwinding enzyme identified as the Ku autoantigen.
8031790 DNA-dependent ATPase from HeLa cells is related to human Ku autoantigen.
8605992 Non-histone protein 1 (NHP1) is a member of the Ku protein family which is upregulated in differentiating mouse myoblasts and human promyelocytes.
8621488 The interaction between Ku antigen and REF1 protein mediates negative gene regulation by extracellular calcium.
10026262 DNA-dependent protein kinase phosphorylation sites in Ku 70/80 heterodimer.
11493912 Structure of the Ku heterodimer bound to DNA and its implications for double-strand break repair.
12145306 Regulation of osteocalcin gene expression by a novel Ku antigen transcription factor complex.
14702039 Complete sequencing and characterization of 21,243 full-length human cDNAs.
14704337 DNA-dependent protein kinase (DNA-PK) phosphorylates nuclear DNA helicase II/RNA helicase A and hnRNP proteins in an RNA-dependent manner.
15075319 Positive and negative modulation of the transcriptional activity of the ETS factor ESE-1 through interaction with p300, CREB-binding protein, and Ku 70/86.
15489334 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
15561718 Systematic identification and analysis of mammalian small ubiquitin-like modifier substrates.
15758953 Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage.
17353262 APLF (C2orf13) is a novel human protein involved in the cellular response to chromosomal DNA strand breaks.
17396150 A novel human AP endonuclease with conserved zinc-finger-like motifs involved in DNA strand break responses.
19608861 Lysine acetylation targets protein complexes and co-regulates major cellular functions.
19690332 Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.
20383123 Ku is a 5'-dRP/AP lyase that excises nucleotide damage near broken ends.
21269460 Initial characterization of the human central proteome.
22002106 Systematic analysis of protein pools, isoforms, and modifications affecting turnover and subcellular localization.
22266820 The E3 ligase RNF8 regulates KU80 removal and NHEJ repair.
22442688 Deformed epidermal autoregulatory factor-1 (DEAF1) interacts with the Ku70 subunit of the DNA-dependent protein kinase complex.
23186163 Toward a comprehensive characterization of a human cancer cell phosphoproteome.
24275569 An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.
24610814 A human short open reading frame (sORF)-encoded polypeptide that stimulates DNA end joining.
25114211 Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli.
25574025 DNA repair. PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair.
25670504 Interactome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway.
25852083 DNA-dependent protein kinase (DNA-PK) permits vascular smooth muscle cell proliferation through phosphorylation of the orphan nuclear receptor NOR1.
25941166 XLS (c9orf142) is a new component of mammalian DNA double-stranded break repair.
25944712 N-terminome analysis of the human mitochondrial proteome.
26359349 Heat shock factor 1, an inhibitor of non-homologous end joining repair.
26502055 The RNF138 E3 ligase displaces Ku to promote DNA end resection and regulate DNA repair pathway choice.
28112733 Site-specific mapping of the human SUMO proteome reveals co-modification with phosphorylation.
28712728 HEXIM1 and NEAT1 Long non-coding RNA form a multi-subunit complex that regulates DNA-mediated innate immune response.
28959974 Regulation of DNA repair pathway choice in S and G2 phases by the NHEJ inhibitor CYREN.
No results found.
Domain Name Domain ID Source
A32626 A35051 PIR
D42397 D42397 PIR
VWF_A IPR002035 InterPro
Ku_C IPR005160 InterPro
Ku_N IPR005161 InterPro
Ku70/Ku80_beta-barrel_dom IPR006164 InterPro
Ku_PK_bind IPR014893 InterPro
SPOC-like_C_dom_sf IPR016194 InterPro
Ku80 IPR024193 InterPro
vWFA_dom_sf IPR036465 InterPro
Ku_C_sf IPR036494 InterPro
Ku PF02735 Pfam
Ku_C PF03730 Pfam
Ku_N PF03731 Pfam
Ku_PK_bind PF08785 Pfam
Ku80 PIRSF016570 PIRSF
S62889 S62889 PIR
Ku78 SM00559 SMART
SSF100939 SSF100939 SUPFAM
SSF101420 SSF101420 SUPFAM
SSF53300 SSF53300 SUPFAM