Protein: Q96B02

UniprotKB AC UniprotKB ID Gene name Full name Species Curated set
Q96B02 (Uniprot) UBE2W_HUMAN UBE2W Ubiquitin-conjugating enzyme E2 W human No
Uniprot: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:20061386). Specifically monoubiquitinates the N-terminus of various substrates, including ATXN3, MAPT/TAU, POLR2H/RPB8 and STUB1/CHIP, by recognizing backbone atoms of disordered N-termini (PubMed:23560854, PubMed:23696636, PubMed:25436519). Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway (PubMed:19111657, PubMed:21229326). In vitro catalyzes 'Lys-11'-linked polyubiquitination. UBE2W-catalyzed ubiquitination occurs also in the presence of inactive RING/U-box type E3s, i.e. lacking the active site cysteine residues to form thioester bonds with ubiquitin, or even in the absence of E3, albeit at a slower rate (PubMed:25436519). more..
MoonDB Dissimilar Functions
GO ID 1 Function 1 Module ID 1 GO ID 2 Function 2 Module ID 2 Association Probability (PrOnto) Interaction Probability (PrOnto)
GO:0009056 catabolic process 125 GO:0009059 macromolecule biosynthetic process 270 6.37e-04 1.88e-40
Biological Processes
Module ID (MoonGO) GO ID (BP) GO Name
53 GO:0006139 nucleobase-containing compound metabolic process
53 GO:0016567 protein ubiquitination
53 GO:0060255 regulation of macromolecule metabolic process
125 GO:0044248 cellular catabolic process
125 GO:0016567 protein ubiquitination
125 GO:0060255 regulation of macromolecule metabolic process
175 GO:0044267 cellular protein metabolic process
175 GO:0006139 nucleobase-containing compound metabolic process
175 GO:0010468 regulation of gene expression
270 GO:0034645 cellular macromolecule biosynthetic process
270 GO:0010467 gene expression
270 GO:0090304 nucleic acid metabolic process
270 GO:0032446 protein modification by small protein conjugation
270 GO:0060255 regulation of macromolecule metabolic process
270 GO:0007165 signal transduction
359 GO:0044248 cellular catabolic process
359 GO:0000209 protein polyubiquitination
359 GO:0007165 signal transduction
375 GO:0070647 protein modification by small protein conjugation or removal
375 GO:0060255 regulation of macromolecule metabolic process
575 GO:0016567 protein ubiquitination
575 GO:0060255 regulation of macromolecule metabolic process
Cellular Components
Module ID (MoonGO) GO ID (CC) GO Name
53 GO:0005829 cytosol
53 GO:0005654 nucleoplasm
125 GO:0005829 cytosol
125 GO:0005634 nucleus
175 GO:0005829 cytosol
175 GO:0005634 nucleus
270 GO:0005829 cytosol
270 GO:0070013 intracellular organelle lumen
270 GO:0005634 nucleus
359 GO:0005634 nucleus
375 GO:0005829 cytosol
375 GO:0005634 nucleus
575 GO:0005654 nucleoplasm
GO ID (BP) GO Name Evidence Code (GO EC)
GO:0006281 DNA repair IEA
GO:0006513 protein monoubiquitination IMP
GO:0006515 protein quality control for misfolded or incompletely synthesized proteins ISS
GO:0016567 protein ubiquitination TAS
GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process IBA
GO:0070979 protein K11-linked ubiquitination IDA
GO:0071218 cellular response to misfolded protein ISS
GO ID (CC) GO Name Evidence Code (GO EC)
GO:0005634 nucleus IBA
GO:0005654 nucleoplasm TAS
GO:0005737 cytoplasm IBA
No pairs of PrOnto dissimilar CC GO terms found.
PMID Article Title
14702039 Complete sequencing and characterization of 21,243 full-length human cDNAs.
15489334 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
16368532 Cloning, characterization and subcellular localization of a gene encoding a human Ubiquitin-conjugating enzyme (E2) homologous to the Arabidopsis thaliana UBC-16 gene product.
16421571 DNA sequence and analysis of human chromosome 8.
19111657 Mechanistic insight into site-restricted monoubiquitination of FANCD2 by Ube2t, FANCL, and FANCI.
20061386 The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines.
21229326 UBE2W interacts with FANCL and regulates the monoubiquitination of Fanconi anemia protein FANCD2.
22496338 A human ubiquitin conjugating enzyme (E2)-HECT E3 ligase structure-function screen.
23560854 Ube2W conjugates ubiquitin to alpha-amino groups of protein N-termini.
23696636 The ubiquitin-conjugating enzyme (E2) Ube2w ubiquitinates the N terminus of substrates.
25436519 Intrinsic disorder drives N-terminal ubiquitination by Ube2w.
No results found.
Domain Name Domain ID Source
UBQ-conjugat_E2 IPR000608 InterPro
UBQ-conjugating_enzyme/RWD IPR016135 InterPro
UQ_con PF00179 Pfam
UBIQUITIN_CONJUGAT_2 PS50127 PROSITE
SSF54495 SSF54495 SUPFAM
UBCc cd00195 CDD