Protein: P06744

UniprotKB AC UniprotKB ID Gene name Full name Species Curated set
P06744 (Uniprot) G6PI_HUMAN GPI Glucose-6-phosphate isomerase human Yes
Uniprot: Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
Dissimilar Functions Manual Curation

Function 1: Glycolytic enzyme. Phosphoglucose isomerase (PGI). Condition: cytosol localisation.

Function 2: Tumor-secreted cytokine. Neuroleukin, autocrine mobility factor, nerve growth factor, differentiation and maturation mediator. Condition: extracellular localisation.

Publications (PMID): 8639816, 15877277, 3020690, 8674049, 12714060

Biological Processes
No results found.
Cellular Components
Module ID (MoonGO) GO ID (CC) GO Name
190 GO:0005829 cytosol
190 GO:0043232 intracellular non-membrane-bounded organelle
GO ID (BP) GO Name Evidence Code (GO EC)
GO:0001525 angiogenesis IEA
GO:0001701 in utero embryonic development IEA
GO:0001707 mesoderm formation IEA
GO:0005975 carbohydrate metabolic process NAS
GO:0006094 gluconeogenesis TAS
GO:0006959 humoral immune response TAS
GO:0007599 hemostasis TAS
GO:0007611 learning or memory IEA
GO:0010469 regulation of receptor activity IEA
GO:0019242 methylglyoxal biosynthetic process IEA
GO:0032355 response to estradiol IEA
GO:0032570 response to progesterone IEA
GO:0033574 response to testosterone IEA
GO:0034101 erythrocyte homeostasis IEA
GO:0035902 response to immobilization stress IEA
GO:0035994 response to muscle stretch IEA
GO:0042593 glucose homeostasis IEA
GO:0043154 negative regulation of cysteine-type endopeptidase activity involved in apoptotic process IEA
GO:0043278 response to morphine IEA
GO:0043312 neutrophil degranulation TAS
GO:0043524 negative regulation of neuron apoptotic process IEA
GO:0046185 aldehyde catabolic process IEA
GO:0046686 response to cadmium ion IEA
GO:0051024 positive regulation of immunoglobulin secretion IDA
GO:0051156 glucose 6-phosphate metabolic process IEA
GO:0061621 canonical glycolysis TAS
GO ID (CC) GO Name Evidence Code (GO EC)
GO:0005576 extracellular region TAS
GO:0005654 nucleoplasm IDA
GO:0005829 cytosol TAS
GO:0005886 plasma membrane IDA
GO:0016020 membrane IDA
GO:0034774 secretory granule lumen TAS
GO:0043005 neuron projection IEA
GO:0043209 myelin sheath IEA
GO:0060170 ciliary membrane IEA
GO:0070062 extracellular exosome IDA
GO:1904813 ficolin-1-rich granule lumen TAS
GO ID 1 Component 1 GO ID 2 Component 2 Association Probability (PrOnto) Interaction Probability (PrOnto)
GO:0005576 extracellular region GO:0005654 nucleoplasm 6.62e-72 7.68e-98
GO:0005654 nucleoplasm GO:0005886 plasma membrane 9.14e-199 6.17e-196
GO:0005654 nucleoplasm GO:0016020 membrane 5.76e-255 1.32e-206
GO:0005654 nucleoplasm GO:0070062 extracellular exosome 3.37e-20 1.23e-35
Interactor Also a MoonDB EMF protein
SPICE_HUMAN Yes: Q8N0Z3 (MoonDB)
PMID Article Title
2387591 Characterization of the 5' end of the gene for human glucose phosphate isomerase (GPI).
3352745 Mouse glucose-6-phosphate isomerase and neuroleukin have identical 3' sequences.
7989588 The characterization of gene mutations for human glucose phosphate isomerase deficiency associated with chronic hemolytic anemia.
8499925 DNA sequence abnormalities in human glucose 6-phosphate isomerase deficiency.
8822952 Study of the molecular defects in glucose phosphate isomerase-deficient patients affected by chronic hemolytic anemia.
8822954 Molecular analysis of glucose phosphate isomerase deficiency associated with hereditary hemolytic anemia.
9446754 Glucosephosphate isomerase (GPI) deficiency mutations associated with hereditary nonspherocytic hemolytic anemia (HNSHA).
9856489 Molecular basis of neurological dysfunction coupled with haemolytic anaemia in human glucose-6-phosphate isomerase (GPI) deficiency.
10727272 Cloning of a glucose phosphate isomerase/neuroleukin-like sperm antigen involved in sperm agglutination.
11004567 Phosphohexose isomerase/autocrine motility factor/neuroleukin/maturation factor is a multifunctional phosphoprotein.
11371164 The crystal structure of human phosphoglucose isomerase at 1.6 A resolution: implications for catalytic mechanism, cytokine activity and haemolytic anaemia.
11437381 Tumor autocrine motility factor is an angiogenic factor that stimulates endothelial cell motility.
12054796 Inhibition mechanism of cytokine activity of human autocrine motility factor examined by crystal structure analyses and site-directed mutagenesis studies.
12163179 Species specificity of the cytokine function of phosphoglucose isomerase.
12573240 Crystal structure of human phosphoglucose isomerase and analysis of the initial catalytic steps.
12777791 The structure of human phosphoglucose isomerase complexed with a transition-state analogue.
14702039 Complete sequencing and characterization of 21,243 full-length human cDNAs.
15057824 The DNA sequence and biology of human chromosome 19.
15489334 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
15637053 Differential regulation of phosphoglucose isomerase/autocrine motility factor activities by protein kinase CK2 phosphorylation.
16139798 Proteomic identification of proteins conjugated to ISG15 in mouse and human cells.
18669648 A quantitative atlas of mitotic phosphorylation.
19413330 Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.
19608861 Lysine acetylation targets protein complexes and co-regulates major cellular functions.
19690332 Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.
21269460 Initial characterization of the human central proteome.
21908771 The first identification of lysine malonylation substrates and its regulatory enzyme.
23186163 Toward a comprehensive characterization of a human cancer cell phosphoproteome.
24275569 An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.
25944712 N-terminome analysis of the human mitochondrial proteome.
OMIM ID Disease Name
613470 Hemolytic anemia, non-spherocytic, due to glucose phosphate isomerase deficiency
Domain Name Domain ID Source
A35333 A35333 PIR
G6P_Isomerase IPR001672 InterPro
Phosphoglucose_isomerase_CS IPR018189 InterPro
G6P_Isomerase_C IPR023096 InterPro
SIS_PGI_1 IPR035476 InterPro
SIS_PGI_2 IPR035482 InterPro
G6P_isomerase MF_00473 HAMAP
PGI PF00342 Pfam
G6PISOMERASE PR00662 PRINTS
P_GLUCOSE_ISOMERASE_2 PS00174 PROSITE
P_GLUCOSE_ISOMERASE_1 PS00765 PROSITE
P_GLUCOSE_ISOMERASE_3 PS51463 PROSITE
PTHR11469 PTHR11469 PANTHER
SIS_PGI_1 cd05015 CDD
SIS_PGI_2 cd05016 CDD