Protein: Q6PDN3

UniprotKB AC UniprotKB ID Gene name Full name Species Curated set
Q6PDN3 (Uniprot) MYLK_MOUSE Mylk Myosin light chain kinase, smooth muscle mouse No
Uniprot: Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains. Involved in the inflammatory response (e.g. apoptosis, vascular permeability, leukocyte diapedesis), cell motility and morphology, airway hyperreactivity and other activities relevant to asthma. Required for tonic airway smooth muscle contraction that is necessary for physiological and asthmatic airway resistance. Necessary for gastrointestinal motility. Implicated in the regulation of endothelial as well as vascular permeability, probably via the regulation of cytoskeletal rearrangements. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis. Plays a role in the regulation of epithelial cell survival. Required for epithelial wound healing, especially during actomyosin ring contraction during purse-string wound closure. Mediates RhoA-dependent membrane blebbing. Triggers TRPC5 channel activity in a calcium-dependent signaling, by inducing its subcellular localization at the plasma membrane. Promotes cell migration (including tumor cells) and tumor metastasis. PTK2B/PYK2 activation by phosphorylation mediates ITGB2 activation and is thus essential to trigger neutrophil transmigration during acute lung injury (ALI). May regulate optic nerve head astrocyte migration. Probably involved in mitotic cytoskeletal regulation. Regulates tight junction probably by modulating ZO-1 exchange in the perijunctional actomyosin ring. Mediates burn-induced microvascular barrier injury; triggers endothelial contraction in the development of microvascular hyperpermeability by phosphorylating MLC. Essential for intestinal barrier dysfunction. Mediates Giardia spp.-mediated reduced epithelial barrier function during giardiasis intestinal infection via reorganization of cytoskeletal F-actin and tight junctional ZO-1. Necessary for hypotonicity-induced Ca(2+) entry and subsequent activation of volume-sensitive organic osmolyte/anion channels (VSOAC) in cervical cancer cells. more..
MoonDB Dissimilar Functions
GO ID 1 Function 1 Module ID 1 GO ID 2 Function 2 Module ID 2 Association Probability (PrOnto) Interaction Probability (PrOnto)
GO:0006355 regulation of transcription, DNA-templated 1677 GO:0022610 biological adhesion 2014 4.83e-02 3.44e-15
Biological Processes
Module ID (MoonGO) GO ID (BP) GO Name
1431 GO:0060255 regulation of macromolecule metabolic process
1507 GO:0070887 cellular response to chemical stimulus
1507 GO:0060255 regulation of macromolecule metabolic process
1523 GO:0006355 regulation of transcription, DNA-templated
1621 GO:0016477 cell migration
1621 GO:0044267 cellular protein metabolic process
1621 GO:0070887 cellular response to chemical stimulus
1621 GO:0120036 plasma membrane bounded cell projection organization
1621 GO:0051336 regulation of hydrolase activity
1626 GO:0031328 positive regulation of cellular biosynthetic process
1626 GO:0010557 positive regulation of macromolecule biosynthetic process
1626 GO:0045935 positive regulation of nucleobase-containing compound metabolic process
1626 GO:0006357 regulation of transcription from RNA polymerase II promoter
1677 GO:0045893 positive regulation of transcription, DNA-templated
1677 GO:0006357 regulation of transcription from RNA polymerase II promoter
1749 GO:0006464 cellular protein modification process
1813 GO:0016477 cell migration
1813 GO:0022607 cellular component assembly
1813 GO:0044267 cellular protein metabolic process
1813 GO:0071310 cellular response to organic substance
1813 GO:0120036 plasma membrane bounded cell projection organization
1813 GO:0031325 positive regulation of cellular metabolic process
1813 GO:0010628 positive regulation of gene expression
1813 GO:0051173 positive regulation of nitrogen compound metabolic process
1943 GO:0022607 cellular component assembly
1943 GO:0034613 cellular protein localization
1943 GO:0071310 cellular response to organic substance
1943 GO:0045184 establishment of protein localization
1943 GO:0010467 gene expression
1943 GO:0046907 intracellular transport
1943 GO:0120036 plasma membrane bounded cell projection organization
1943 GO:0006468 protein phosphorylation
1943 GO:0050790 regulation of catalytic activity
2014 GO:0007155 cell adhesion
2014 GO:0016477 cell migration
2014 GO:0044267 cellular protein metabolic process
2014 GO:0071310 cellular response to organic substance
2014 GO:0022604 regulation of cell morphogenesis
2014 GO:0120035 regulation of plasma membrane bounded cell projection organization
2014 GO:0007165 signal transduction
Cellular Components
Module ID (MoonGO) GO ID (CC) GO Name
1431 GO:0044428 nuclear part
1523 GO:0005634 nucleus
1602 GO:0031982 vesicle
1621 GO:0030054 cell junction
1621 GO:0009986 cell surface
1621 GO:0070062 extracellular exosome
1621 GO:0030027 lamellipodium
1626 GO:0044428 nuclear part
1677 GO:0031981 nuclear lumen
1749 GO:0044430 cytoskeletal part
1749 GO:0005634 nucleus
1813 GO:0030054 cell junction
1813 GO:0009986 cell surface
1813 GO:0070062 extracellular exosome
1813 GO:0120025 plasma membrane bounded cell projection
1813 GO:0044459 plasma membrane part
1943 GO:0015629 actin cytoskeleton
1943 GO:0044430 cytoskeletal part
1943 GO:0030027 lamellipodium
2014 GO:0030054 cell junction
2014 GO:0009986 cell surface
2014 GO:0044430 cytoskeletal part
2014 GO:0070062 extracellular exosome
2014 GO:0120025 plasma membrane bounded cell projection
2014 GO:0044459 plasma membrane part
GO ID (BP) GO Name Evidence Code (GO EC)
GO:0006468 protein phosphorylation IEA
GO:0006939 smooth muscle contraction IMP
GO:0014820 tonic smooth muscle contraction IMP
GO:0030335 positive regulation of cell migration ISO
GO:0032060 bleb assembly ISO
GO:0035690 cellular response to drug IDA
GO:0035865 cellular response to potassium ion IDA
GO:0051928 positive regulation of calcium ion transport ISO
GO:0060414 aorta smooth muscle tissue morphogenesis ISO
GO:0071277 cellular response to calcium ion IDA
GO:0071476 cellular hypotonic response ISO
GO:0090303 positive regulation of wound healing ISO
GO ID (CC) GO Name Evidence Code (GO EC)
GO:0001725 stress fiber ISO
GO:0005737 cytoplasm ISO
GO:0005911 cell-cell junction IDA
GO:0030027 lamellipodium ISO
GO:0032154 cleavage furrow ISO
GO:0070062 extracellular exosome ISO
No pairs of PrOnto dissimilar CC GO terms found.
PMID Article Title
11029314 Smooth muscle myosin light chain kinase expression in cardiac and skeletal muscle.
11121372 Telokin expression is restricted to smooth muscle tissues during mouse development.
11472067 The myosin light chain kinase gene is not duplicated in mouse: partial structure and chromosomal localization of Mylk.
11832329 220- and 130-kDa MLCKs have distinct tissue distributions and intracellular localization patterns.
12360480 Intestinal infection with Giardia spp. reduces epithelial barrier function in a myosin light chain kinase-dependent fashion.
15489334 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
16055522 Deletion of MLCK210 induces subtle changes in vascular reactivity but does not affect cardiac function.
16141072 The transcriptional landscape of the mammalian genome.
16184195 Epithelial myosin light chain kinase-dependent barrier dysfunction mediates T cell activation-induced diarrhea in vivo.
17242355 Large-scale phosphorylation analysis of mouse liver.
17577141 A role for long chain myosin light chain kinase (MLCK-210) in microvascular hyperpermeability during severe burns.
17925381 Supervillin slows cell spreading by facilitating myosin II activation at the cell periphery.
18586037 Myosin light chain kinase is central to smooth muscle contraction and required for gastrointestinal motility in mice.
18587400 Nonmuscle myosin light-chain kinase mediates neutrophil transmigration in sepsis-induced lung inflammation by activating beta2 integrins.
20018858 Myosin light chain kinase is necessary for tonic airway smooth muscle contraction.
20404178 MLCK-dependent exchange and actin binding region-dependent anchoring of ZO-1 regulate tight junction barrier function.
21074048 A family of protein-deglutamylating enzymes associated with neurodegeneration.
21183079 A tissue-specific atlas of mouse protein phosphorylation and expression.
No results found.
Domain Name Domain ID Source
Prot_kinase_dom IPR000719 InterPro
Ig_sub2 IPR003598 InterPro
Ig_sub IPR003599 InterPro
FN3_dom IPR003961 InterPro
Ig-like_dom IPR007110 InterPro
Ser/Thr_kinase_AS IPR008271 InterPro
Kinase-like_dom_sf IPR011009 InterPro
Ig_I-set IPR013098 InterPro
Ig-like_fold IPR013783 InterPro
MLCK1_kinase_dom IPR015725 InterPro
Protein_kinase_ATP_BS IPR017441 InterPro
FN3_sf IPR036116 InterPro
Ig-like_dom_sf IPR036179 InterPro
fn3 PF00041 Pfam
Pkinase PF00069 Pfam
I-set PF07679 Pfam
PROTEIN_KINASE_ATP PS00107 PROSITE
PROTEIN_KINASE_ST PS00108 PROSITE
PROTEIN_KINASE_DOM PS50011 PROSITE
IG_LIKE PS50835 PROSITE
FN3 PS50853 PROSITE
FN3 SM00060 SMART
S_TKc SM00220 SMART
IGc2 SM00408 SMART
IG SM00409 SMART
SSF48726 SSF48726 SUPFAM
SSF49265 SSF49265 SUPFAM
SSF56112 SSF56112 SUPFAM
FN3 cd00063 CDD
STKc_MLCK1 cd14191 CDD